DESCRIPTIONS OF MEDICAL FUNGI

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20 Descriptions of Medical Fungi Aspergillus lentulus Balajee & Marr Antifungal Susceptibility: A. lentulus (Australian National data); MIC µg/mL. No 16 AmB 5 1 1 3 VORI 5 3 2 POSA 5 1 1 3 ITRA 5 2 1 1 1 Neosartorya fischeri (Wehmer) Malloch & Cain Neosartorya fischeri is mostly found in canned foodstuffs and is now documented as a causative agent of invasive aspergillosis in immunosuppressed patients. RG-1 organism. Morphological Description: Colonies of N. fischeri are suede-like to floccose, white to pale yellow with slow to poor conidiation. Conidial heads are short, columnar and uniseriate. Conidiophore stipes are smooth-walled and vesicles are usually subglobose to flask-shaped. Conidia globose to subglobose (2-2.5 µm in diameter), smooth to finely roughened. Good growth at 37 O C. a b b 10 μm 10 μm Neosartorya fischeri (a) culture and (b) conidial head morphology.
Descriptions of Medical Fungi 21 Neosartorya fischeri (Wehmer) Malloch & Cain Molecular Identification: N. fischeri can be distinguished from other members of the section Fumigati by sequence analysis of β-tubulin, calmodulin and actin genes (Samson et al. 2007; Balajee et al. 2005b). ITS sequencing is not recommended. Antifungal Susceptibility: N. fischeri (Australian National data); MIC µg/mL. No 16 AmB 8 1 5 2 VORI 8 1 3 3 1 POSA 8 4 1 1 2 ITRA 8 1 2 2 3 Aspergillus nidulans complex Aspergillus subgenus Nidulantes; Gams et al. (1985) includes species with biseriate conidial heads, brown pigmented often short stipes, and green conidia. Cleistothecia are soft-walled, surrounded by Hülle cells, and ascospores are red to purple in colour. Section Nidulantes is one of the largest subgenera of the genus Aspergillus, and includes about 80 species. Several species have been reported as medical pathogens principally Aspergillus nidulans, but also A. sydowii, A. unguis, A. rugulovalvus and A. tetrazonus. Molecular Identification: ITS sequencing is sufficient to identify to species complex only. A. nidulans can be distinguished from other members of the section Nidulantes by sequence analysis of β-tubulin, calmodulin and actin genes. Aspergillus nidulans (Eidam) Wint. Aspergillus nidulans is a typical soil fungus with a worldwide distribution, it has also been reported to cause disease in human and animals. RG-1 organism. Morphological Description: On Czapek Dox agar, colonies are typically plain green in colour with dark red-brown cleistothecia developing within and upon the conidial layer. Reverse may be olive to drab-grey or purple-brown. Conidial heads are short, columnar (up to 70 x 30 µm in diameter) and biseriate. Conidiophore stipes are usually short, brownish and smooth-walled. Conidia are globose (3-3.5 µm in diameter) and rough-walled. Key Features: Conidial heads are short, columnar and biseriate. Stipes are usually short, brownish and smooth-walled. Conidia are globose and rough-walled.
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DESCRIPTIONS OF MEDICAL FUNGI

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