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CO2 Sequestration through Deep Saline Injection and ...

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Scale-Up<br />

Knowing how growth rates <strong>and</strong> energetic parameters are affected by scale up <strong>and</strong> developing a<br />

basis of comparison between reactor types is important when developing an industrial process.<br />

For a PBR, there are two important parameters in comparison between reactors <strong>and</strong> in scaleup:<br />

the light energy supplied per unit volume (Io/V), <strong>and</strong> the light distribution coefficient (Kiv) [30].<br />

It is obvious that the greater the light supplied per unit volume, the higher the rate will be. It<br />

should also be obvious that for constant illumination area, cellular concentration, <strong>and</strong> volume,<br />

that the growth rate will be linear below the saturation light intensity <strong>and</strong> will fall away from<br />

linear in proportion to Es in Eq. (1) above the saturation intensity. However, it is also important<br />

how the light is distributed within the reactor. This is something that varies with reactor type.<br />

The light distribution coefficient is a measure of how the light is distributed <strong>and</strong> is defined as the<br />

cell concentration at which 50% of the PBR volume received enough light to have positive<br />

cellular yield [29]. The light intensity at which no net growth occurs can be found from Eq. (6)<br />

by setting Y equal to zero. The volume that is above that light intensity can be found using Eqs<br />

(2) <strong>and</strong> (3). The percentage can then be calculated from that volume. The concentration will<br />

have to be varied until the fraction is 0.5, this concentration is taken as Kiv. Figure 4 shows this<br />

concept for two different types of reactors.<br />

Figure 4<br />

Light Distribution Coefficient, Conceptual Example [30]<br />

Because Kiv is higher for the lower reactor type in Figure 4 even though the total light<br />

illumination is the same, the rate is higher because the sustained cellular concentration is higher.<br />

It was shown that the growth rate is linear with respect to both Io/V <strong>and</strong> Kiv [30]. Thus a single<br />

coefficient, the light supply coefficient (Io/V*Kiv), is a measure that can be used for comparison<br />

between reactor types <strong>and</strong> for scale up [30]. That is to say, if data is collected in one reactor<br />

type in the lab, it can be used to size <strong>and</strong> design a full scale reactor of any other type or size as<br />

long as the light supply coefficient is the same between them. This is shown in Figure 5. The<br />

linear portion of the growth rate vs light supply coefficient is linear because it falls below the<br />

saturation light intensity. Equation (1) will have to be incorporated into the growth rate above<br />

the saturation intensity. It is important to note that at high light intensities, Et becomes constant<br />

with respect to light intensity at approximately 0.03 rather than 0.2 at low light intensities. It is<br />

because of this that two linear or nearly linear regions exist in Figure 5. A low light supply<br />

coefficient corresponds to low light intensity <strong>and</strong> vice versa. It would be expected that the rate<br />

would be linear with respect to light supply coefficient until the point of light saturation <strong>and</strong> that<br />

14

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