108139-1 Study Title Data Requirements Author Study ... - IR-4 Project

Bayer Corporation 108139-1
Agriculture Division
The standard concentration is equal to the ppm relative to matrix weight in an
undiluted sample taking into account the initial sample size, aliquoting of the
original sample, and the final extract volume. The following equation may be
used to calculate the ppm equivalence of the standard solution concentration.
S x F x T
Standard conc. (ppm equivalence) =
W x A
where 6 $QDO\WLFDO VWDQGDUG FRQFHQWUDWLRQ J P/
F = Final extract volume (mL),
T = Total initial extract volume (mL),
W = Initial target sample weight (g),
A = Aliquot taken
An example sample residue calculation showing the use of the above formulae is presented in
Appendix 2.
D. Detector Linearity Curves
1. To demonstrate linearity of the response factor of cyfluthrin versus the
corresponding [ 2 H6]cyfluthrin internal standard in either solvent or matrix extract,
a series of 7 different concentrations were used. More points were sometimes
needed depending on the amount of residue measured.
Transfer 150 / IURP D FRQWURO H[WUDFW FRQWDLQLQJ QR > 2 H6]cyfluthrin standard into
7 different auto sampler vials. Evaporate the solutions to dryness. Then transfer
/ RI WKH DSSURSULDWH VWDQGDUG WKDW FRQWDLQV F\IOXWKULQ DQG > 2 H6]cyfluthrin into
each of the 7 auto sampler vials to yield the solvent + matrix containing curve.
7UDQVIHU / RI WKH DSSURSULDWH VWDQGDUG LQWR HDFK RI DQRWKHU DXWR VDPSOHU
vials to yield the solvent only curve. The contents of the vials are shown below:
Vial 1 Blank 0.00000 J P/ F\IOXWKULQ J P/ > 2 H6]cyfluthrin
Vial 2 0.002 ppm 0.0020 J P/ F\IOXWUKLQ J P/ > 2 H6]cyfluthrin
Vial 3 0.004 ppm 0.0040 J P/ F\IOXWUKLQ J P/ > 2 H6]cyfluthrin
Vial 4 0.010 ppm 0.010 J P/ F\IOXWUKLQ J P/ > 2 H6]cyfluthrin
Vial 5 0.020 ppm 0.020 J P/ F\IOXWKULQ J P/ > 2 H6]cyfluthrin
Vial 6 0.050 ppm 0.050 J P/ F\IOXWKULQ J P/ > 2 H6]cyfluthrin
Vial 7 0.100 ppm 0.10 J P/ F\IOXWKULQ J P/ > 2 H6]cyfluthrin
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