COVID19 Pandemic

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In a new study, published in the journal Experimental
Neurobiology, the team developed a protocol optimized
for cheap and easy wide-scale detection of SARS-CoV-
2/COVID-19 based on sample self-collection from the
tested individual via pharyngeal swab, Trizol-based RNA
purification and SYBR Green-based RT-PCR. This protocol
was specifically designed with conditionsoptimized
for testing of asymptomatic individuals. The
self-collection of tissue samples from the tonsils via
pharyngeal swab in this protocol was performed to prevent
contact between the volunteers participating in the
study, as well as between volunteers and the health care
professionals conducting the test. The collected tissue
on swab was extracted with commercially available
Trizol kit, whereby the application of the Trizol reagent
led to denaturation and inactivation of the virus particles
and all following steps of the procedure could be
performed in a conventional biosafety level 2 laboratory.
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