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COVID19 Pandemic

COVID 19 Pandemic, Group RDM

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78

he next step of the protocol also involved the application

of a commercial kit to convert the virus

RNA to cDNA. For the following quantitative RT-PCR

step, primer pairs were designed based on the published

full sequence of SARS-CoV-2/COVID-19 and the

reaction was performed with the use of SYBR Green

PCR Master Mix. SARS-CoV-2/COVID-19 total RNA

was used as a positive control and determination of

detection efficiency and limit were done using lentivirus

(a well-known, RNA-containing research-purpose

recombinant virus). The lentivirus tests revealed an accuracy

and sensitivity limit of 1–10 virus particles for

the newly established protocol

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