COVID19 Pandemic
COVID 19 Pandemic, Group RDM
COVID 19 Pandemic, Group RDM
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he next step of the protocol also involved the application
of a commercial kit to convert the virus
RNA to cDNA. For the following quantitative RT-PCR
step, primer pairs were designed based on the published
full sequence of SARS-CoV-2/COVID-19 and the
reaction was performed with the use of SYBR Green
PCR Master Mix. SARS-CoV-2/COVID-19 total RNA
was used as a positive control and determination of
detection efficiency and limit were done using lentivirus
(a well-known, RNA-containing research-purpose
recombinant virus). The lentivirus tests revealed an accuracy
and sensitivity limit of 1–10 virus particles for
the newly established protocol
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