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ACKNOWLEDGMENTS This thesis ends a three years period of doctoral studies in the Laboratory of Plasma Electronics and Engineering at Saga University, Japan. Obviously, I could not have done and finish this work without the help of several important people. First of all, I want to express my profound gratitude to Professor Hiroharu Fujita for the unique opportunity he has offered me to come and work in his laboratory. I am especially grateful for the total freedom he gave me in research, always ready to support, guide and discuss scientific, as well general aspects of Japanese daily life. A particular thank goes here to Associate Professor Yasunori Ohtsu for his kindness and enthusiasm showed in the last three years, who has dedicated a lot of his time, despite of a extremely busy schedule, for reviewing and commenting all my entire scientific activity. I am indebted to the other members of the referee committee Professor Kat- sunori Shida, Professor Satoru Goto and Professor Kazuhiro Muramatsu for their helpful criticism and availability during the redaction of this manuscript. Next, I would like to thank to Dr. Tatsuya Misawa with whom I had many discussions, always constructive and helpful. More than that, I want to show ii
my appreciation especially for the technical magic he has done in the workshop helping me, especially when a tight time schedule was pressing. Very special thanks go to Dr. Sebastian Popescu, my office colleague in the firsttwoyearsandahalfatSagaUniversity, for the all the things he contributed to this important period of my life. Also, it is time here to thank for the help and support that Dr. Popescu provided me to go through the difficult period of integration from Romanian to Japanese culture and life style. You have been always a good friend. At the end of these three years, I can proudly report that I “know how to swim” on my own. Nonetheless, as a sign of my total appreciation, I would like to dedicate this entire work to Viorel and Maria Vicoveanu, my parents who have succeeded in the difficult task of motivating me to stay in shape and never loose the faith, providing me unconditional support and love. Also, to my brother Bogdan, it is time now to dedicate this thesis for encouraging me all these years, especially when dark clouds were above me. iii
Page 1: PLASMA STERILIZATION USING LOW PRES
Page 5 and 6: Iis ion saturation current [µA] t
Page 7 and 8: CONTENTS 1 PRINCIPLES OF SURFACE DI
Page 9 and 10: 4 EFFECTS OF LOW TEMPERATURE PULSED
Page 11 and 12: and intuited to bring new important
Page 13 and 14: Figure 1.1: Schematic representatio
Page 15 and 16: Table 1.1: Common disinfection and
Page 17 and 18: the radiation energy is resonantly
Page 19 and 20: ers of heat, optical radiation and
Page 21 and 22: Table 1.2: Main plasma sources used
Page 23 and 24: microorganism population as a respo
Page 25 and 26: UV Photon Before After DNA Figure 1
Page 27 and 28: to sterilize medical tools, but the
Page 29 and 30: sented by both theoretical and expe
Page 31 and 32: Plasma Sterilization Using Low Pres
Page 33 and 34: Figure 2.1: Experimental set-up. MB
Page 35 and 36: Figure 2.3: Electronic circuit of t
Page 37 and 38: such circumstances, the inactivatio
Page 39 and 40: ment time t is N = N0e −αt ≡ N
Page 41 and 42: shows the number of the viable micr
Page 43 and 44: has n −1 0 > 281.25 µm 2 availab
Page 45 and 46: 1 st 2 nd ( I) TNTC (II) TNTC (III)
Page 47 and 48: U LC blocking filter 19.74 pF 20.24
Page 49 and 50: Figure 2.8: Experimental set-up use
Page 51 and 52: the reactive species from plasma ar
Page 53 and 54:
Temperature measurement Relative in
Page 55 and 56:
MR MP SH Figure 3.2: Experimental a
Page 57 and 58:
a fast temperature increasing rate.
Page 59 and 60:
D h (min) 10 3 10 2 10 1 10 0 100 2
Page 61 and 62:
MR QP SH Figure 3.6: Experimental a
Page 63 and 64:
The contribution of photons and hea
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D (min) 10 3 10 2 10 1 10 0 Dν D h
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N/N 0 (%) 10 2 10 2 10 2 10 2 10 2
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D (min) 10 2 10 1 10 0 D h+ν+p D h
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holder is mainly given by particle
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3.5 Power sensitivity of spore inac
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If for a reference treatment temper
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of the sample was achieved. Moreove
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microorganisms were disposed in mon
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The pulse period covered a wide int
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in chapter 2. The treatment time of
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4.2.2 Injected RF power In order to
Page 87 and 88:
ton =500µs, and the duty cycle was
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T ( 0 C) 120 100 80 60 40 20 I 0 5
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N/N 0 (%) 10 2 10 2 10 2 10 1 10 1
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elow 70 ◦ C. 3. Increasing the in
Page 95 and 96:
the second phase of the inactivatio
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k 1, 2 (min -1 ) 1.2 1.0 0.8 0.6 0.
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I (arb. unit) 140 120 100 80 60 40
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I (arb. unit) 5 4 3 2 1 0 0.000 0.0
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I > (arb.unit) 1.0 0.9 0.8 0.7 0.6
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shown in Fig. 4.14. It can be seen
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I is (mA) 0.25 0.20 0.15 0.10 0.05
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value. When ton has been kept const
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survival curve characteristics were
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of the effects produced by differen
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solutely necessary for a profound u
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Conferences 1. Bacillus spores ster
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[9] W. A. Rutala, D. J. Weber, J. H
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[34] J. H. Young: Sterilization wit
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[57] M. K. Boudam, M. Moisan, B. Sa
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[79] J. Schneider, K. M. Baumgartne
Page 127 and 128:
[100] S. Villeger, S. Cousty, A. Ri
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