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List of Symbols A surface of the glass slide [mm 2 ] Aeff effective contaminated area of the glass slide [mm 2 ] AV average number of colonies CFU colony forming units D decimal reduction values [min] DF dilution factor Di Dh Dh+ν D h+ν+p Dν Dp decimal reduction factor due to the action of the i inactivation agent [min] decimal inactivation agent due to the heat action [min] decimal reduction value due to the actions of heat and photons [min] decimal reduction value due to the plasma action [min] decimal inactivation agent due to the photon action [min] decimal reduction value due to the plasma particles action [min] e electron charge 1.602 × 10 −19 C e logarithm natural base E energy [W] fp pulse frequency [Hz] k inactivation rate [min −1 ] ki inactivation rate due to the action of the i inactivation agent [min −1 ] I intensity of the light plasma emission [arb. unit] iv
Iis ion saturation current [µA] time averaged emission of the light plasma emission [arb. unit] time averaged value of the ion saturation current [A] M ion mass [kg] n0 ne mean number of spores per effective contaminated areea plasma density [m −3 ] N number of the individuals within a microorganisms population N0 initial number of the individuals within a microorganisms population N(t) number of surviving microorganisms at the time t N(t + ∆t) number of surviving microorganisms at the time t + ∆t P radio-frequency power [W] P cw eff P pp eff effective continuous wave injected RF power [W] effective pulsed injected RF power [W] P pulse RF power injected during the pulse [W] P pulse eff effective RF power injected during the pulse [W] r microorganisms inactivation speed [units×min −1 ] S probe collection surface [mm 2 ] t time [min] toff ton T period [min] Te power off time period [min] power on time period [min] electron temperature [<strong>eV</strong>] v
Page 1 and 2: PLASMA STERILIZATION USING LOW PRES
Page 3: my appreciation especially for the
Page 7 and 8: CONTENTS 1 PRINCIPLES OF SURFACE DI
Page 9 and 10: 4 EFFECTS OF LOW TEMPERATURE PULSED
Page 11 and 12: and intuited to bring new important
Page 13 and 14: Figure 1.1: Schematic representatio
Page 15 and 16: Table 1.1: Common disinfection and
Page 17 and 18: the radiation energy is resonantly
Page 19 and 20: ers of heat, optical radiation and
Page 21 and 22: Table 1.2: Main plasma sources used
Page 23 and 24: microorganism population as a respo
Page 25 and 26: UV Photon Before After DNA Figure 1
Page 27 and 28: to sterilize medical tools, but the
Page 29 and 30: sented by both theoretical and expe
Page 31 and 32: Plasma Sterilization Using Low Pres
Page 33 and 34: Figure 2.1: Experimental set-up. MB
Page 35 and 36: Figure 2.3: Electronic circuit of t
Page 37 and 38: such circumstances, the inactivatio
Page 39 and 40: ment time t is N = N0e −αt ≡ N
Page 41 and 42: shows the number of the viable micr
Page 43 and 44: has n −1 0 > 281.25 µm 2 availab
Page 45 and 46: 1 st 2 nd ( I) TNTC (II) TNTC (III)
Page 47 and 48: U LC blocking filter 19.74 pF 20.24
Page 49 and 50: Figure 2.8: Experimental set-up use
Page 51 and 52: the reactive species from plasma ar
Page 53 and 54: Temperature measurement Relative in
Page 55 and 56:
MR MP SH Figure 3.2: Experimental a
Page 57 and 58:
a fast temperature increasing rate.
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D h (min) 10 3 10 2 10 1 10 0 100 2
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MR QP SH Figure 3.6: Experimental a
Page 63 and 64:
The contribution of photons and hea
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D (min) 10 3 10 2 10 1 10 0 Dν D h
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N/N 0 (%) 10 2 10 2 10 2 10 2 10 2
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D (min) 10 2 10 1 10 0 D h+ν+p D h
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holder is mainly given by particle
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3.5 Power sensitivity of spore inac
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If for a reference treatment temper
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of the sample was achieved. Moreove
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microorganisms were disposed in mon
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The pulse period covered a wide int
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in chapter 2. The treatment time of
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4.2.2 Injected RF power In order to
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ton =500µs, and the duty cycle was
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T ( 0 C) 120 100 80 60 40 20 I 0 5
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N/N 0 (%) 10 2 10 2 10 2 10 1 10 1
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elow 70 ◦ C. 3. Increasing the in
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the second phase of the inactivatio
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k 1, 2 (min -1 ) 1.2 1.0 0.8 0.6 0.
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I (arb. unit) 140 120 100 80 60 40
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I (arb. unit) 5 4 3 2 1 0 0.000 0.0
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I > (arb.unit) 1.0 0.9 0.8 0.7 0.6
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shown in Fig. 4.14. It can be seen
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I is (mA) 0.25 0.20 0.15 0.10 0.05
Page 109 and 110:
value. When ton has been kept const
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survival curve characteristics were
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of the effects produced by differen
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solutely necessary for a profound u
Page 117 and 118:
Conferences 1. Bacillus spores ster
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[9] W. A. Rutala, D. J. Weber, J. H
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[34] J. H. Young: Sterilization wit
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[57] M. K. Boudam, M. Moisan, B. Sa
Page 125 and 126:
[79] J. Schneider, K. M. Baumgartne
Page 127 and 128:
[100] S. Villeger, S. Cousty, A. Ri
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ε (eV)

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