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RF O MB VP PG GT SH DC LP BG to pumps Figure 2.2: Photographic view of plasma experimental set-up. O-oscilloscope. MB-matching box. VP-voltage probe. RF-radio frequency source. GT-gas tank. SH-spores holder. LP-Langmuir probe. DC-discharge chamber. BG-baratron gauge. ment is presented in Fig. 2.1. The height of the reactor is 150 mm and the diameter is 300 mm. The upper part of the vessel can be lifted, giving access to the spore holder (SH in Fig. 2.1). The detailed picture of the experimental device can be seen in the Fig. 2.2 The plasma diagnosis and observation was realized through a few plexiglas ports and one quartz window. The RF power was injected into the plasma by the help of the horizontal, internal ring antenna, 200 mm in diameter, made of a 25
Figure 2.3: Electronic circuit of the matching box. stainless-steel tube, and covered with an electrical insulator. The antenna was located in the center of the chamber, and was connected, through a manual matching box unit (MB in Fig. 2.1), to a 13.56 MHz RF power supply. The detailed view of the matching network can be seen in Fig. 2.3. The input power to plasma can be optimized by acting on the two variable condensers (Fig. 2.3) in order to minimize the RF power loses in the system. Therefore, the power injected in the system can be found subtracting the reflected power from the incident power (i.e., the power from the output of the RF source). These can be visualized on the main electronic front panels of the VTC−0HI10 model B023 RF source. The support for the spore sample was a horizontal stainless steel Petri dish, 8 cm in diameter, situated at 3.5 cm below the horizontal plane of the antenna, on the vertical symmetry axis of the reactor. During the experiments this holder was 26
Page 1 and 2: PLASMA STERILIZATION USING LOW PRES
Page 3 and 4: my appreciation especially for the
Page 5 and 6: Iis ion saturation current [µA] t
Page 7 and 8: CONTENTS 1 PRINCIPLES OF SURFACE DI
Page 9 and 10: 4 EFFECTS OF LOW TEMPERATURE PULSED
Page 11 and 12: and intuited to bring new important
Page 13 and 14: Figure 1.1: Schematic representatio
Page 15 and 16: Table 1.1: Common disinfection and
Page 17 and 18: the radiation energy is resonantly
Page 19 and 20: ers of heat, optical radiation and
Page 21 and 22: Table 1.2: Main plasma sources used
Page 23 and 24: microorganism population as a respo
Page 25 and 26: UV Photon Before After DNA Figure 1
Page 27 and 28: to sterilize medical tools, but the
Page 29 and 30: sented by both theoretical and expe
Page 31 and 32: Plasma Sterilization Using Low Pres
Page 33: Figure 2.1: Experimental set-up. MB
Page 37 and 38: such circumstances, the inactivatio
Page 39 and 40: ment time t is N = N0e −αt ≡ N
Page 41 and 42: shows the number of the viable micr
Page 43 and 44: has n −1 0 > 281.25 µm 2 availab
Page 45 and 46: 1 st 2 nd ( I) TNTC (II) TNTC (III)
Page 47 and 48: U LC blocking filter 19.74 pF 20.24
Page 49 and 50: Figure 2.8: Experimental set-up use
Page 51 and 52: the reactive species from plasma ar
Page 53 and 54: Temperature measurement Relative in
Page 55 and 56: MR MP SH Figure 3.2: Experimental a
Page 57 and 58: a fast temperature increasing rate.
Page 59 and 60: D h (min) 10 3 10 2 10 1 10 0 100 2
Page 61 and 62: MR QP SH Figure 3.6: Experimental a
Page 63 and 64: The contribution of photons and hea
Page 65 and 66: D (min) 10 3 10 2 10 1 10 0 Dν D h
Page 67 and 68: N/N 0 (%) 10 2 10 2 10 2 10 2 10 2
Page 69 and 70: D (min) 10 2 10 1 10 0 D h+ν+p D h
Page 71 and 72: holder is mainly given by particle
Page 73 and 74: 3.5 Power sensitivity of spore inac
Page 75 and 76: If for a reference treatment temper
Page 77 and 78: of the sample was achieved. Moreove
Page 79 and 80: microorganisms were disposed in mon
Page 81 and 82: The pulse period covered a wide int
Page 83 and 84: in chapter 2. The treatment time of
Page 85 and 86:
4.2.2 Injected RF power In order to
Page 87 and 88:
ton =500µs, and the duty cycle was
Page 89 and 90:
T ( 0 C) 120 100 80 60 40 20 I 0 5
Page 91 and 92:
N/N 0 (%) 10 2 10 2 10 2 10 1 10 1
Page 93 and 94:
elow 70 ◦ C. 3. Increasing the in
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the second phase of the inactivatio
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k 1, 2 (min -1 ) 1.2 1.0 0.8 0.6 0.
Page 99 and 100:
I (arb. unit) 140 120 100 80 60 40
Page 101 and 102:
I (arb. unit) 5 4 3 2 1 0 0.000 0.0
Page 103 and 104:
I > (arb.unit) 1.0 0.9 0.8 0.7 0.6
Page 105 and 106:
shown in Fig. 4.14. It can be seen
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I is (mA) 0.25 0.20 0.15 0.10 0.05
Page 109 and 110:
value. When ton has been kept const
Page 111 and 112:
survival curve characteristics were
Page 113 and 114:
of the effects produced by differen
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solutely necessary for a profound u
Page 117 and 118:
Conferences 1. Bacillus spores ster
Page 119 and 120:
[9] W. A. Rutala, D. J. Weber, J. H
Page 121 and 122:
[34] J. H. Young: Sterilization wit
Page 123 and 124:
[57] M. K. Boudam, M. Moisan, B. Sa
Page 125 and 126:
[79] J. Schneider, K. M. Baumgartne
Page 127 and 128:
[100] S. Villeger, S. Cousty, A. Ri
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