canping-huang-phd-novel-virus-discovery-in-bat-isn-translation

Figure 1.5 The identification of the reorganized p10 gene and its TRS. (A) Confirmation of the “foreign”
p10 gene. The upstream sequence spanning the junction of the N gene and the p10 gene and the downstream
junction of the p10 gene and the Ns7a gene are shown in the figure. The length of the spacer sequence
between the N gene and the p10 gene is denoted by a number. The TRS of the NS7a gene is marked with a red
arrow located within the spacer sequence between the genes. (B) The identification of the p10 gene TRS.
The TRS of the p10 gene within the N gene coding sequence is shown in the figure. The number indicates the
distance between the start codon of the p10 gene and its TRS. Numbers indicate the length of the spacer
sequence between the N gene and its downstream gene. The TRS of the gene downstream from the N gene is
marked with a red arrow.
Figure 1.6 The length of the spacer sequence between the end of the N gene and the TRS of the gene
downstream from it, in different virus genomes. N represents the nucleocapsid gene. TRS means
transcription regulatory sequence. The number between N and TRS is the length of the spacer sequence.
Noticeably, the 3’ end of the N gene in Ro-BatCoV GCCDC1 is truncated (Figure 1.7). The
integration of the “foreign ” p10 gene disrupts the open reading frame of the N gene. As a
result, the 3’ end is missing 8 amino acids and two amino acids were deleted in the
neighboring area.
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A partial translation into English of the PhD thesis: “Novel Virus Discovery in Bat and the Exploration of
Receptor of Bat Coronavirus HKU9” by Canping Huang, Chinese Center for Disease Control and Prevention,
2016. Translation completed for Independent Science News in March 2021.