canping-huang-phd-novel-virus-discovery-in-bat-isn-translation

The other was from a Miniopterus fuliginosus spleen (101-4x). (Figure 3.1)
Figure 3.1 Testing for Coronavirus and Agarose Gel Electrophoresis Analysis. The numbers above the
images are the order number used during testing. 109-2 and 101-4x are the actual sample numbers. As
indicated by the red arrow, the positive bands are the expected size. The DNA molecular weight standards are:
2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp.
Two samples appear to be bocavirus positive: One liver and one lung sample from
Rhinolophus sinicus (B13-3). The other was a spleen sample from Rhinolophus sinicus
(B122-4). (Figure 3.2)
Figure 3.2 Testing for Bocavirus and Agarose Gel Electrophoresis. The numbers above the images are the
order number used during testing. B13-3 and 122-4 are the sample numbers. As indicated by the red arrow,
the positive bands are the expected size. The DNA molecular weight standards are: 2000 bp, 1000 bp, 750 bp,
500 bp, 250 bp, and 100 bp.
[11]* samples appear to be astrovirus positive: five bowel samples (B110-2, B135-2, B12-2,
B124-2 and B129-2); three liver and lung samples (B12-3, B124-3 and B43-3) and one
spleen sample (B124-4) from Rhinolophus sinicus (Figure 3.3).
* This should say “9 samples appear to be astrovirus positive”, the error is in the original PhD.
79
A partial translation into English of the PhD thesis: “Novel Virus Discovery in Bat and the Exploration of
Receptor of Bat Coronavirus HKU9” by Canping Huang, Chinese Center for Disease Control and Prevention,
2016. Translation completed for Independent Science News in March 2021.