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Figure 3.3 Testing for Astrovirus and Agarose Gel Electrophoresis. The numbers above the figures are

the order number used during testing. B110-2, B135-2, B12-2, B124-2, B129-2, B12-3, B124-3, B43-4 and

B124-4 are the sample numbers. As indicated by the red arrow, the positive bands are the expected size. The

DNA molecular weight standards are: 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp.

3.2.2 Sequence Homology and Phylogenetic Analysis

Figure 3.4 Coronavirus Phylogenetic Analysis. Red circles indicate coronaviruses detected in this research.

Blue diamonds indicate evolutionarily related coronaviruses. Phylogenetic trees were constructed with

MEGA7 software using the neighbor-joining method and a bootstrap value of 1000.

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A partial translation into English of the PhD thesis: “Novel Virus Discovery in Bat and the Exploration of

Receptor of Bat Coronavirus HKU9” by Canping Huang, Chinese Center for Disease Control and Prevention,

2016. Translation completed for Independent Science News in March 2021.

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