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Figure 1.5 The identification of the reorganized p10 gene and its TRS. (A) Confirmation of the “foreign”p10 gene. The upstream sequence spanning the junction of the N gene and the p10 gene and the downstreamjunction of the p10 gene and the Ns7a gene are shown in the figure. The length of the spacer sequencebetween the N gene and the p10 gene is denoted by a number. The TRS of the NS7a gene is marked with a redarrow located within the spacer sequence between the genes. (B) The identification of the p10 gene TRS.The TRS of the p10 gene within the N gene coding sequence is shown in the figure. The number indicates thedistance between the start codon of the p10 gene and its TRS. Numbers indicate the length of the spacersequence between the N gene and its downstream gene. The TRS of the gene downstream from the N gene ismarked with a red arrow.Figure 1.6 The length of the spacer sequence between the end of the N gene and the TRS of the genedownstream from it, in different virus genomes. N represents the nucleocapsid gene. TRS meanstranscription regulatory sequence. The number between N and TRS is the length of the spacer sequence.Noticeably, the 3’ end of the N gene in Ro-BatCoV GCCDC1 is truncated (Figure 1.7). Theintegration of the “foreign ” p10 gene disrupts the open reading frame of the N gene. As aresult, the 3’ end is missing 8 amino acids and two amino acids were deleted in theneighboring area.41A partial translation into English of the PhD thesis: “Novel Virus Discovery in Bat and the Exploration ofReceptor of Bat Coronavirus HKU9” by Canping Huang, Chinese Center for Disease Control and Prevention,2016. Translation completed for Independent Science News in March 2021.
Figure 1.7 Comparison of the nucleotides and amino acid sequences at the terminus of gene N. As theresult shows, the 3’ end of the N gene in Ro-BatCoV GCCDC1 is truncated.[Translator’s Note: The sections starting from 1.2.7 SubgenomicStructures of Ro-BatCoV GCCDC1 (starting p. 42) through section 2.2.1Expression and Purification of of HKU9-RBD (ending p. 62) were nottranslated into English]42A partial translation into English of the PhD thesis: “Novel Virus Discovery in Bat and the Exploration ofReceptor of Bat Coronavirus HKU9” by Canping Huang, Chinese Center for Disease Control and Prevention,2016. Translation completed for Independent Science News in March 2021.
Page 1 and 2: This document is the partial transl
Page 3 and 4: that are present in the body of the
Page 5 and 6: A partial translation into English
Page 7 and 8: Declaration of Dissertation Origina
Page 11 and 12: 3.1.5 Primer Design……………
Page 19 and 20: IntroductionCoronaviruses are envel
Page 21 and 22: [Translator’s Note: The sections
Page 23: Figure 1.4 The continuity and integ
Page 27 and 28: However, the external subdomain is
Page 29 and 30: 3.1 Materials and Methods3.1.1 Reag
Page 31 and 32: Explanation: All N’s were replace
Page 33 and 34: Figure 3.3 Testing for Astrovirus a
Page 35 and 36: Similarly, sequence homology result
Page 37 and 38: Part Two: Literature Review85[Trans
Page 39 and 40: these two viruses have led to human
Page 41 and 42: Bats are one of the most ancient ma
Page 43 and 44: The organs, therefore, do not have
Page 45 and 46: In 2013, a study by Ge et al. stron
Page 47 and 48: Chapter Six: Technology Used to Ide
Page 49 and 50: For instance, Vero cells are interf
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Page 53 and 54: Articles PublishedA partial transla
Page 55 and 56: Xiang Nan Ren, Chun Feng Yun, Hai T

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