Shimadzu Techno-Research Inc., Japan - UNEP Chemicals

�� DMSO/Hex. partioning��effective partioning effective for air & sediment
mineral oil & aliphatic hydrocarbons
�� Ultra high level congeners��major congeners major PCB, p,p‘-DDE, p,p DDE,
p,p’-DDD p,p DDD saturated peak, memory effects in the GC
column, separate analysis for different analyte with
cleanup factor
�� Blank of POPs�� POPs HCB��Chlordane HCB Chlordane & BFR(HBB, DeBDE),
endsulfan, great care should be taken for the
laboratory dust
�� HCB & chlordane has relatively high method blank
level��pg level pg��on on the other hand low level in the biological
sample
SHIMADZU TECHNO RESEARCH, Inc.
Experiences of sample for POPs analysis
by HRGC/HRMS
Insufficient cleanup ��
�� Broad GC peak shape : dirty Ion source & liner
�� Variation of ionization efficiency, sensitivity, change
of ion intensity of fragment ions, variation of monitor
ions, degradation & adsorption in the GC column
�� cleanup is most important for HRMS analysis
Unstable POPs affect variation of sensitivity :
Endrin, Endrin,
Heptachlor, DDT
�� Strongly recommended use of 13 13C C internal standards,
standards
but variation of sensitivity and recovery % should
occur even though stable RRF
�� interference of PBDE(Tetra) to PCB(Penta) on HT-8 HT
GC column
SHIMADZU TECHNO RESEARCH, Inc.