PELONEWS Autumn 2023

PELONEWS
WITH A FOCUS ON ORGANOID RESEARCH
PELOBiotech Newsletter Autumn 2023
"Science is a way of
thinking much more
than it is a body of
knowledge."
– Carl Sagan,
American astronomer
and science
communicator
Dear Scientist,
Organoids, miniature 3D organs, are transforming cellular research and
opening new therapeutic avenues. Mimicking human tissues, they enable
vital cell communication and offer insights into disease progression. With
potential as drug delivery systems, organoids are revolutionizing
therapeutics.
Within organoids, diverse cell types interact, facilitating the transport of
essential lipids, proteins, and nucleic acids between cells. This dynamic
network of cellular crosstalk drives tissue development, disease
progression, and potential therapeutic interventions.
Remember to take advantage of our exclusive 10% back to lab
discount on selected products. Simply mention it when placing your
order, and the discount will be applied.
All the best to you and your research,
Christiane Büchsel
————————————————————————
PELOAcademy | Brain organoid models
• Organoids
CONTENT
• Media for MSCs
• Why is defined media better
for Organoids
• Organ-on-a-Chip
• Dermal Papilla cell media
• New Products
• New Primary Cell list
presented by Dr. Insa Schröder,
GSI Helmholtz Zentrum für Schwerionenforschung on October 24, 2023,
10 AM.Check out our website for further details:
https://www.pelobiotech.com/webinars.html

Overview Organoids
PELONews Autumn 2023
Reprinted from “Organoid Generation from Pluripotent Stem Cells”, by BioRender.com (2023).
Organoids are three-dimensional
miniaturized organ-like structures that are
derived from stem cells or tissue samples.
They are composed of multiple cell types
and closely mimic the structure and
function of specific organs or tissues in the
body.
Organoids offer a unique platform for
studying organ development, disease
modelling, and personalized medicine.
They provide a more accurate
representation of human biology
compared to traditional two-dimensional
cell cultures, allowing researchers to
investigate complex cellular interactions
and responses to various stimuli.
Organoids hold great promise in
advancing our understanding of organ
physiology, drug discovery, and
regenerative medicine.
PELONews: Organoids, Unlocking the power of 3D Tissue
Models for Disease Research and Drug Discovery
Scan/Click the QR code to read our article to find out.
Why is defined media better for organoids?
• Ethics: FBS is derived from the blood of unborn calves, raising ethical concerns regarding animal
welfare. In contrast, defined media eliminate the need for FBS, reducing reliance on animalderived
components. .
• Consistency: Defined media ensures consistent and reproducible manufacturing as it is
precisely formulated.
• Control: Precisely formulated composition provides control over exosome production and
thereby improves scalability and efficiency in manufacturing
• Optimization: Tailoring media composition enhances exosome yield, purity, and functionality.
• Standardization: Eliminating undefined components like serum ensures standardized exosome
preparation and uniform reproducible results.
• Safety: Minimizing contaminants and unwanted effects with defined media ensures high-quality
exosomes by eliminating adverse immune reactions associated with undefined components..
• Compliance: Regulatory guidelines and ethical considerations are met in exosome production.
Defined media reduce the risk of potential contaminants or unwanted biological effects. By
precisely defining the media composition, unwanted variations and impurities can be minimized,
ensuring the production of high-quality organoids.
2 I www.pelobiotech.com

Overview Organoids
Table 1. Comparison of Cell Culture Factors for Cell Growth and Exosome Studies. Adapted from
Liau et al, Int J Mol Sci, 2021
Factors FBS hPL Defined Media EXO Free Media
Pros
Abundant and
accessible
Easy to produce
Well-defined
components
Exosome-depleted and ideal for
isolation
Suitable for most cells Suitable for most cells No batch-to-batch
variation
No batch-to-batch variation
Supports cell survival
and proliferation
Supports cell survival
and proliferation
No risk of xenogeneic/
allogeneic protein
contamination
No risk of xenogeneic/allogeneic
protein contamination
Widely used in studies
and publications
No risk of xenogenic
immune reaction; can
be produced using
autologous blood
No unintended
interaction with test
substances
No unintended interaction with test
substances
Cons
Components are illdefined
Components are illdefined
Not available for
certain cells
Not available for certain cells
Batch-to-batch
variation
Batch-to-batch
variation (reduced by
pooling)
Some media require
extra coating for cell
attachment
Some media require extra coating
for cell attachment
Potential
contamination (prions,
mycoplasma, bacteria,
fungi)
Unintended interaction
with test substances
More expensive
compared to
alternatives
More expensive compared to
alternatives
Risk of animal protein
contamination in
clinical usage
Few distributors
Time-consuming and
difficult to develop
Time-consuming and difficult to
develop
Unintended interaction
with test substances
Potential allogenic
immune response
Higher particle count
and presence of
exosomes
Ethical concerns with
animal welfare
Risk of transmitting
human viruses
Multitude of exosomes
not suitable for
isolation or studies
Multitude of exosomes
not suitable for
isolation or studies
3

Overview Organoids
PELONews Autumn 2023
Reprinted from “Application of Organoids”, by BioRender.com (2023).
Reprinted from “Protocol to Culture Primary Human Basal Epithelial Cells in 2D and 3D System”, by BioRender.com
4 I www.pelobiotech.com

Overview Organoids
Extracellular matrix
MEGAROBO ExtraGel matrix offers a versatile hydrogel matrix
derived from basement membrane components found in ECMrich
mouse tumour tissues. It is composed of laminin I, collagen
IV, and heparan sulfate proteoglycans, providing a supportive
environment enriched with growth factors such as epidermal
growth factor, platelet-derived growth factor, nerve growth
factor, basic fibroblast growth factor, transforming growth
factor, and insulin growth factors. This matrix is available in large lot sizes and can be obtained with
or without growth factors, along with options for phenol red or reduced growth factors. It has
been rigorously tested for applications in 3D cell culture, angiogenesis, stem cell maintenance,
differentiation, and in vivo xenograft generation, making it a valuable tool for a wide range of
research and clinical applications.
Media and supplements for MSCs
To optimize exosome isolation from MSCs, we advise using tailored products at distinct stages,
including isolation, maintenance, proliferation, and exosome isolation. Our recommendations are
Human Platelet Lysate (hPL) and defined media. Check out our last newsletter to find more details
about the same.
CellCor
MSC CD
AOF
Made with BioRender 2023
Animal origin-free chemically defined media for human-derived mesenchymal
stem cells growth and proliferation composed of synthetic and recombinant
proteins, rigorously tested for bioactivity, endotoxin, mycoplasma, and more.
CellCor
EXO CD
Exosome
depleted
UltraGRO
PURE GI
Chemically defined media for stable MSC growth from different tissues, enabling
high-quality exosome production. Versatile and compatible with various methods
for consistent, reproducible results. Ideal for research and clinical applications.
Promotes low aging and high genetic stability in MSCs from Adipose, Bone
marrow, Cord blood, and Wharton jelly.
Xeno-free, Exosome-depleted, gamma-irradiated, and fibrinogen-depleted hPL
available for research, clinical, and commercial use.
5

PELONews Autumn 2023
Overview Organ-on-a-chip
Organoids, and organ-on-a-chip, have transformed the landscape of
biomedical research and clinical applications.
Organ-on-a-chip platforms replicate the complex microenvironment of
organs, integrating multiple cell types and physiological functions onto a
single chip.
These systems enable researchers to study organ-level responses, drug
interactions, and disease mechanisms with high precision.
The combination of organoids and organ-on-a-chip technology offers a
powerful approach to simulate human organ systems and bridge the gap
between traditional in vitro models and animal testing.
Made with BioRender 2023
This advancement not only enhances our understanding of human biology
and disease but also contributes to the development of safer and more
effective treatments, while reducing reliance on animal models. The FDA's
recognition of these technologies by no longer requiring animal testing
reinforces the importance of embracing human-relevant models and ethical
alternatives for advancing healthcare and scientific discoveries.
Read our article by scanning/clicking the QR code to find out more.
Intestine-on-a-Chip for Personalized Medicine
Reprinted from “Intestine-on-a-chip”, by BioRender.com (2023).
Intestine-on-a-chip models also facilitate the study of gastrointestinal diseases, e.g. inflammatory
bowel disease, by mimicking the inflammatory responses and barrier dysfunction observed in
these conditions.
PELONews: New organelle discovered in an animal cell
Scan/Click the QR code to read our article here to find out.
6 I www.pelobiotech.com

Overview Organ-on-a-chip
Precision-Cut Tissue Slices: Enhancing In Vivo Research and Results
Made with BioRender 2023
Precision-cut lung, heart, and brain slices are valuable tools
used in research to study various aspects of these organs in a
controlled and detailed manner as they also preserve in-vivo
niches. These slices are thin sections of tissues obtained
through specialized techniques and equipment, ensuring
minimal damage to the tissue's architecture and cellular
structure.
They can help in disease modelling, drug testing and
development, toxicology studies, pharmacokinetics studies,
electrophysiological studies, imaging and histology, and
disease biomarker discovery.
These slices maintain the tissue's native architecture, allowing
researchers to study organs in a physiologically relevant three
-dimensional context. Cellular interactions and complexity are preserved,
mimicking the in vivo environment more accurately. Organ specific functions,
such as air exchange, electrical activity, and contractility, are retained, enhancing
the fidelity of research findings. Furthermore, precision-cut slices enable the
investigation of drug permeability and interactions across natural barriers within
organs. Compared to cell cultures, these slices reduce experimental artefacts and
offer more realistic pharmacokinetic data and are more ethical making them a
valuable tool in various research applications.
Want to test out precision cut tissue slices? Scan the QR code to contact us for
more details!
Apricell 3-in-1 plate
To study co-culture
Tumour invasion
ECM study
Drug screening
Scilia ExtraGel ECM
Reconstituted matrix hydrogel
3D cell culture
Angiogenesis
Stem cell maintenance and
differentiation
In-vivo xenograft generation
Organoid kits
With cell culture medium and
cytokines to create cardiac,
biliary, breast, brain, lung,
liver, vascular organoids
Organoid products in R&D quality
• Organoid kits with cytokines, media and cells included for culture of organoids
• Recombinant human laminins for various cell types, including cardiomyocytes (iMatrix-211),
endothelial cells (iMatrix-411), pluripotent stem cells (iMatrix-511), corneal epithelial cells
(iMatrix-332), and hepatocytes (iMatrix-111). iMatrix-511 is also available in a ready-to-use
formulation for your convenience.
• Scilia ExtraGel ECM equivalent to the gold-standard in the industry.
• Apricell 3-in-1 plate for high-throughput drug testing.
Scan/click the QR code to learn about out ECM for 2D & 3D cell culture.
7

PELONews Spring/Summer Autumn 2023 2023
PELOAcademy
Join us for an exciting new webinar on brain
organoids scheduled for October 24, 10 AM.
Ensure your participation by becoming a
PELOClub member by scanning the QR code.
Don't miss out!
PELOIntern
Under the warm embrace
of summer, PELOBiotech
gathered at the
biergarden of Haderner
Augustiner for a
memorable summer party.
The rustic charm,
delectable Bavarian
cuisine, and lively
conversation created an
ambiance of pure joy and
togetherness. The evening
strengthened bonds and
left everyone with a
renewed sense of camaraderie that would linger long after the
summer sun had set.
We are happy to provide you with additional
information and product details upon request.
Simply scan the QR code and complete the form.
Also, meet our CEO, Dr. Peter Frost, at SelectBio
conference 2023 on November 13+14 in Rotterdam, NL, and
Medica, November 15+16 in Düsseldorf.
How to reach us
If you need any further
assistance or if you like
what you see, tell us:-)
PELOBiotech GmbH
Klopferspitz 19
82152 Planegg
Germany
+49 89 517 286 59 0
info@pelobiotech.com
www.pelobiotech.com
Managing Directors:
Dr. Peter Frost,
Dr. Lothar Steeb
You like what you
see?
Sign up for PELONews
at
www.pelobiotech.com
or just fill out the online
form
Fill out our Portfolio and
send it back to us.
info@pelobiotech.com
Or
give us a call
+49 89 517 286 59-0
Welcome aboard! New faces at PELOBiotech
Neyla
Cassandra
Shinson
We had a fantastic time at the
EuroTox 2023, meeting new people
and we hope to soon meet those
who couldn't make it.
Working Student
Sales Executive
Working Student
8 I www.pelobiotech.com