PELONEWS Autumn 2023

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Overview Organoids PELONews Autumn 2023 Reprinted from “Application of Organoids”, by BioRender.com (2023). Reprinted from “Protocol to Culture Primary Human Basal Epithelial Cells in 2D and 3D System”, by BioRender.com 4 I www.pelobiotech.com
Overview Organoids Extracellular matrix MEGAROBO ExtraGel matrix offers a versatile hydrogel matrix derived from basement membrane components found in ECMrich mouse tumour tissues. It is composed of laminin I, collagen IV, and heparan sulfate proteoglycans, providing a supportive environment enriched with growth factors such as epidermal growth factor, platelet-derived growth factor, nerve growth factor, basic fibroblast growth factor, transforming growth factor, and insulin growth factors. This matrix is available in large lot sizes and can be obtained with or without growth factors, along with options for phenol red or reduced growth factors. It has been rigorously tested for applications in 3D cell culture, angiogenesis, stem cell maintenance, differentiation, and in vivo xenograft generation, making it a valuable tool for a wide range of research and clinical applications. Media and supplements for MSCs To optimize exosome isolation from MSCs, we advise using tailored products at distinct stages, including isolation, maintenance, proliferation, and exosome isolation. Our recommendations are Human Platelet Lysate (hPL) and defined media. Check out our last newsletter to find more details about the same. CellCor MSC CD AOF Made with BioRender 2023 Animal origin-free chemically defined media for human-derived mesenchymal stem cells growth and proliferation composed of synthetic and recombinant proteins, rigorously tested for bioactivity, endotoxin, mycoplasma, and more. CellCor EXO CD Exosome depleted UltraGRO PURE GI Chemically defined media for stable MSC growth from different tissues, enabling high-quality exosome production. Versatile and compatible with various methods for consistent, reproducible results. Ideal for research and clinical applications. Promotes low aging and high genetic stability in MSCs from Adipose, Bone marrow, Cord blood, and Wharton jelly. Xeno-free, Exosome-depleted, gamma-irradiated, and fibrinogen-depleted hPL available for research, clinical, and commercial use. 5
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PELONEWS Autumn 2023

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