tumor in the light of the revised SIOP-01 classification

92
in Figure 5 indicate that combination of BN-PAGE and in-
-gel activity assay can be successfully applied for detection
of individual respiratory chain complex activity deficiencies
(in this case complex IV). As it is presented, only 10 μg of
mitochondria isolated from the skeletal muscle biopsy is sufficient
to measure activity of complex IV using in-gel assay.
Fig. 5 In gel activity assay for the evaluation of complex IV activity in
skeletal muscle mitochondria from healthy volunteer and patient with the
COX deficiency
Perspectives
A great advantage of the combined method of BN-PAGE and
in-gel activity assay is that relatively small quantities of tissue
are required for evaluating the amount and the activity
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Research was supported by Internal Project of The Children’s
Memorial Health Institute Nr 158/06 (principal investigator
– doc. B. Cukrowska), Internal Project of The
Children’s Memorial Health Institute Nr 161/06 (principal
investigator – doc. M. Pronicki) and Grant Nr PB
0890/PO5/2005/29 (principal investigator – prof. E. Pronicka).
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