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Manual for Diagnosis of Screw-worm Fly - xcs consulting

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A <strong>Manual</strong> <strong>for</strong> the <strong>Diagnosis</strong> <strong>of</strong> <strong>Screw</strong>-Worm <strong>Fly</strong><br />

TABLE 1. DIAGnOSTIC CuTICuLAR HyDROCARBOn PEAKS FOR Chrysomya AnD CoChliomyia SPECIES<br />

C. bezziana C. megacephala C. saffranea Co. hominivorax Co. macellaria<br />

Compound1 ECL2 Male Female Male Female Male Female Male Female Female<br />

% % % % % % % % % % % % %<br />

n-C25 25.00 2.1 (0.7) 1.9 (0.5) 0.4 0.3 0.2 0.4 0.9 (9.6) 0.4 (2.1) 0.5<br />

i-MeC25 25.37 0.2 (0.7) 0.2 (0.2) 0.8 1.3 0.7 1.3 0.4 (20.2) 0.0 (0.0) 1.5<br />

2-MeC26 26.64 0.8 (1.5) 0.9 (0.6) 4.2 5.2 4.7 5.3 0.4 (0.0) 0.2 (0.0) 0.6<br />

C27:1 26.79 1.2 (1.1) 1.1 (0.4) 1.8 3.6 3.4 7.7 0.1 (0.0) 0.0 (0.0) 0.0<br />

n-C27 27.00 4.0 (2.2) 3.6 (2.0) 8.3 8.5 6.6 8.5 2.7 (12.0) 1.4 (8.1) 13.8<br />

i-MeC27 27.33 1.1 (1.8) 1.0 (0.8) 18.6 19.4 17.8 17.6 2.5 (6.9) 0.9 (6.8) 20.7<br />

5-MeC27 27.51 0.2 (0.2) 0.2 (0.1) 0.3 0.3 0.3 0.3 0.2 (0.0) 0.1 (0.0) 3.4<br />

diMeC27 27.64 0.1 (0.3) 0.1 (0.1) 4.9 3.8 4.7 3.4 1.4 (0.0) 0.5 (1.4) 5.4<br />

3-MeC27 27.75 2.2 (0.5) 1.9 (0.5) 0.8 0.7 0.7 0.7 2.0 (4.0) 1.5 (2.7) 12.8<br />

2-MeC28 28.64 6.8 (7.8) 6.9 (3.2) 15.2 14.8 14.7 12.3 8.7 (1.6) 9.4 (2.1) 1.7<br />

n-C29 29.00 3.9 (4.2) 3.5 (5.4) 6.4 6.3 6.7 6.1 13.7 (9.6) 12.7 (13.8) 8.5<br />

i-MeC29 29.33 8.4 (5.5) 7.7 (2.6) 8.0 6.8 7.9 6.5 25.9 (4.0) 25.4 (11.0) 7.1<br />

diMeC29 29.63 0.9 (0.7) 0.9 (0.4) 2.5 2.5 3.0 1.9 10.7 (0.9) 10.0 (2.5) 2.0<br />

30MeC29 29.74 1.7 (0.6) 1.6 (0.5) 0.6 0.1 0.4 0.3 5.1 (2.0) 4.1 )3.1) 1.5<br />

2-MeC30 30.64 8.2 (11.6) 9.1 (11.0) 2.1 2.0 1.8 1.9 2.4 (0.7) 3.1 (1.4) 0.3<br />

i-MeC31 31.31 12.3 (7.8) 11.8 (4.0) 3.5 3.7 3.6 3.7 2.6 (1.1) 3.6 (4.5) 1.3<br />

diMeC31 31.57 5.6 (2.5) 5.6 (1.6) 1.1 1.3 2.3 2.0 2.0 (0.0) 2.9 (2.1) 0.3<br />

i-MeC33 33.31 7.0 (7.5) 7.2 (7.0) 1.0 1.1 0.8 1.1 0.2 (2.4) 1.4 (5.2) 0.6<br />

diMeC33 33.57 10.5 (4.4) 10.8 (3.0) 0.7 0.7 0.3 0.7 0.3 (0.0) 1.2 (4.0) 0.0<br />

i-MeC35 35.31 1.3 (5.4) 1.4 (9.4) 0.3 0.2 0.2 0.3 0.0 (0.9) 0.5 (3.3) 0.3<br />

diMeC35 35.57 4.0 (3.5) 4.6 (4.3) 0.0 0.1 0.0 0.1 0.0 (0.0) 0.7 (4.5) 0.2<br />

i-MeC37 37.30 0.1 (2.1) 0.2 (5.1) 0.2 0.1 0.0 0.1 0.0 (0.0) 0.0 (0.0) 0.0<br />

diMeC37 37.54 1.1 (5.2) 1.6 (9.4) 0.1 (0.1) 0.0 0.0 0.0 0.0 0.0 (0.0) 0.0<br />

50<br />

Values are percentages <strong>for</strong> newly-emerged or young flies, except <strong>for</strong> those in parentheses which are <strong>for</strong> 5 day olds.<br />

1 Provisional identification; I-MeC25 represents internal-methylpentacosane. 2 Equivalent chain length. 3 From Promonis (1989), percentages are <strong>of</strong> alkanes only.

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