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Manual for Diagnosis of Screw-worm Fly - xcs consulting

Manual for Diagnosis of Screw-worm Fly - xcs consulting

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A <strong>Manual</strong> <strong>for</strong> the <strong>Diagnosis</strong> <strong>of</strong> <strong>Screw</strong>-Worm <strong>Fly</strong><br />

Measure solids first and add liquids, adding dimethyl disulphide last. It is crucial that the<br />

correct chemicals be used, especially the n-isomers <strong>of</strong> butyric and valeric acids. Care must<br />

be taken when mixing and dispensing s<strong>worm</strong>lure which is very caustic, and has a long-lasting<br />

and unpleasant odour. Store in a refrigerator not used <strong>for</strong> foodstuffs.<br />

The traps are suspended by cords from trees or posts at a height <strong>of</strong> about 1.5m. For<br />

surveillance, maximising the opportunity <strong>for</strong> catching SWF is important and shady sites at<br />

the edge <strong>of</strong> wooded areas, near water sources or cattle camps are recommended. Close<br />

proximity to rubbish dumps or animal carcasses will result in large numbers <strong>of</strong> secondary<br />

blowflies on the traps and these sites should be avoided.<br />

Traps can be serviced at convenient intervals from daily to weekly. Up to 10 days exposure<br />

can be used but trapped flies deteriorate with time. In some locations, <strong>for</strong>aging stingless<br />

bees may remove the adhesive. When serviced, the aluminium plate is replaced and the<br />

s<strong>worm</strong>lure container replenished. When large numbers <strong>of</strong> traps are being serviced, trap<br />

plates can be stored in plywood boxes (35 x 60cm in cross section) <strong>of</strong> sufficient length to<br />

hold the number <strong>of</strong> (paired) plates required.<br />

Flies on the trap plate are removed with <strong>for</strong>ceps and placed on appropriately labelled (locality,<br />

trap number, date, etc.) cards or plastic petri dishes, using the residual adhesive to attach fly<br />

to card or dish. Cards or dishes can be stored in a freezer to await microscopical examination<br />

and identification.<br />

Larva <strong>of</strong> the New World screw-<strong>worm</strong> fly Cochlimyia hominivorax.<br />

Scanning electron microscope photograph by Colin Beaton<br />

56

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