DEPARTMENT OF VETERINARY MEDICINE AND PRIMATE ... - DPZ

DEPARTMENT OF VETERINARY MEDICINE AND
PRIMATE HUSBANDRY
General survey of services
The Department of Veterinary Medicine and Primate Husbandry represents the
central infrastructure unit within the DPZ. As such it is responsible for various services
related to areas of animal breeding, husbandry, veterinary care and animal
protection. These include the purchase and quarantine of imported primates, the
sale of animals from our own breeding colonies, as well as aspects of veterinary care
which are performed internally and externally. During the report year, a total of
2893 animals were treated once or several times, and in vivo samples were obtained
for numerous groups using minimally invasive and non-invasive techniques. Diagnostic
investigations were either carried out by the department itself or co-ordinated
by it. During necropsies, samples were taken from diverse organs for a number of external
user groups. Altogether, 68 necropsies and histological analyses were performed
in the year as a service for internal and external groups. A central electron
microscopy unit with corresponding photographic laboratory is available for external
and internal users. Numerous guided tours as well as training and re- training
measures on the subject of primate care give practical insight into the work of animal
keepers, technicians, biologists and veterinary practitioners.
External services
Advice and guided tours
Name/Institute Service
Individuals, school groups, private groups,
journalists, representatives of Governmental
organisations, scientists and student
groups with up to 150 members from all
sectors of society
Individuals, veterinary practitioners,
representatives of national Governmental
institutions and NGOs, industry, students,
scientists, zoos, press
Samples sold
Department of Veterinary Medicine and Primate Husbandry
25 guided tours of the Department of
Primate Husbandry, including provision
of information, interviews, film opportunities
etc, on general and specific questions
of captive primate care.
67 detailed statements of advice, partly
including literature research, advisory
notes and reports on various aspects of
veterinary care and primate husbandry.
Name/Institute Service
Society for Therapeutic Research,
Nürnberg
450 ml Heparin blood from rhesus monkey
Div. Gastroenterology and Hepatology,
School of Medicine, Hannover
100 ml pooled serum from rhesus monkey
Primate Report 54, July 1999 151

Name/Institute Service
Fa. Biomed. Heidelberg EDTA-blood samples from rhesus monkey
Dept. Nuclear Medicine, University Hospital
Benjamin Franklin, Free Univ. Berlin
200 ml pooled monkey serum
Fa. Hodawand, Bio-Immun-Labordiagnostik
Ltd
Oesophagus, marmoset spp. and rhesus
monkey
Fa. Euroimmun, Lübeck Organ samples from 5 cynomolgus monkeys
Fa. MA BioServices, Heidelberg EDTA-blood samples from rhesus monkey
Samples donated
Department of Veterinary Medicine and Primate Husbandry
Name/Institute Service
Paul-Ehrlich-Institute, Langen
Dr. Beer
2 x 15 ml baboon heparinized blood
Anthropology Dept. Columbia University,
New York, USA
Dr.A.J.Tosi
Dept. Immunogenetics, University of
Göttingen
Prof. Dr. Günther
Eye Clinic, University Hospital Tübingen
PD Dr. J. Kremers
Institute of Zoology, University of
Heidelberg
Bernhard-Nocht-Institute, Hamburg
T. Greve
Bernhard-Nocht-Institute, Hamburg
Prof. Dr. Racz,
Robert-Koch-Institute Berlin
S. Biel
Dept of Anatomy, University of Göttingen
Prof. Dr. Meyer
University of Göttingen
Dr. T. Schulz
5 ml blood from Macaca silenus
Liver, lymph node and skin tissue from a
healthy rhesus monkey and one suffering
from enteritis; intestinal tissue from
2 rhesus monkeys
4 ml blood each from 20 male rhesus
monkeys
Blood samples from rhesus monkeys,
cynomolgus monkeys, lion-tailed monkey
Blood samples from different primate
species (2 squirrel monkeys, 2 common
marmosets, 3 lion-tailed macaques)
Regular sampling of various lymphatic
organs of SIV-infected rhesus monkeys
Urine and blood samples from 3 immunodeficient
rhesus monkeys and 2 cynomolgus
monkeys, kidney tissue from 2 rhesus
monkeys
Eyes of 5 elderly rhesus monkeys originating
from Cayo Santiago, Puerto Rico
Liver, kidney, lung, pancreas of 8 rhesus
monkeys, frozen and fixed in formalin
152 Primate Report 54, July 1999

Department of Veterinary Medicine and Primate Husbandry
Name/Institute Service
University of Leipzig
Prof. Dr. Überla
Lower Saxonian Institute for Peptide
Research, Hannover
Dr. Mägert
University of Würzburg
Dr. Sopper
11 necropsies incl. histological analysis of
immunodeficient rhesus monkeys
Frozen liver tissue from 8 rhesus monkeys
3 necropsies including CNS-Perfusions and
histological analysis in rhesus monkeys
Animal Park Gettorf Necropsy and histological analysis of a
black-tufted ear marmoset (Callithrix
penicillata)
Dept Nephrology, School of Medicine, Skin from 6 marmosets
Hannover
Prof. Dr. Floege,
Veterinary practitioner Kopp Pancreas of 2 rhesus monkeys
Leibniz Research Laboratories for Biotechnology
and Artificial Organs, Hannover
Dr. Martin
Institute for Molecular Virology, University
of Nürnberg-Erlangen
PD Dr. Fickenscher
School of Medicine, Hannover
Dr. Ahrends
Institute of Pathology, School of Veterinary
Medicine, Hannover
Dr. U. Hetzel
Veterinary services performed at cost
Blood samples and vascular endothelias
from squirrel monkeys, common marmosets,
rhesus monkeys, cynomolgus monkeys
Blood samples from squirrel monkeys
Arteries and veins in cell culture medium
from 4 cynomolgus monkeys
Eyes from various primate species
Name/Institute Service
Animal Park Gronau Microbiological, virological and parasitological
analysis of blood and faecal samples
from 12 baboons (in collaboration with
Dept. Pathology)
Animal Park Nordhorn
Dr. Kramer
Microbiological and parasitological analyses
(in collaboration with Dept. Pathology)
Animal Park Daun Clinical analyses including tuberculinization,
sampling for microbiological, parasitological
and haematological analysis of
barbary macaques
Primate Report 54, July 1999 153

Veterinary services donated
Name/Institute Service
Ecopark Frankenau Explantation of a contraceptive in a cottontop
tamarin
Max von Pettenkofer-Institute, München
Prof. Dr. G. Frösner
Histological analysis of ISH-marked brain
and liver sections of a brown-headed
tamarin
Animal Park Dortmund Necropsy and histological analysis of a
squirrel monkey
Endocrinology, Dept. Obstetrics and
Gynecology, University of Göttingen
Dr. I. Knoke
Landesanstalt für veterinärmedizinische
Untersuchungen Ehrental, A
Bayer AG Wuppertal
PD Dr. Rosenbruch
Histological analysis of a porcine Corpora
lutea
Postmortal determination of age in a
squirrel monkey
Histological analysis of liver sections of a
cynomolgus monkey
Ebbs Zoo, A Clinical-ethological and dermato-pathological
analysis of alopecia in a rhesus
monkey colony
Hannover Zoo
Dr. Knüfermann
Institute of Anthropology, University of
Göttingen
Prof. Dr. Rothe
Cell Biology, Laboratory of the Dept.
Obstetrics and Gynecology, University
of Göttingen
PD Dr. Augustin
Robert-Koch-Institute Berlin
Dr. Gelderblom
Immunohistological analysis of SIVexpression
in 3 orang-utan lymphomas
Veterinary care of the Callithrix-colony
Provision of electron microscopy preparations
including semi- and ultrathin
sections for various investigations
Participation in the 6 th open experiment in
electron microscopical viral diagnosis
Zoo Stendal Necropsy and histological analysis of a
senile lion-tailed macaque
Biomedical Primate Research Centre,
Rijswijk, NL
Dr. E.-M. Kuhn,
Nienburg
Dr. Barkhoff
Department of Veterinary Medicine and Primate Husbandry
Wild Animal Park, Springe
Dr. J. Müller
Light- and electron microscopical analysis
of selected cases in primate pathology
Histological analysis of different feline eye
diseases with corresponding histological
documentations
Histological analysis of tissue from different
wild animal species.
154 Primate Report 54, July 1999

Teaching and education
University teaching
F-J. Kaup
• 2-hour seminar, postgraduate studies in pathology, summer term 1998: 15.04.98,
Pathology of the Immunodeficiency Syndrome, School of Veterinary Medicine,
Hannover.
• Lecture series on the special pathology of the liver, 25., 26. and 02.05.98, School of
Veterinary Medicine, Hannover.
• Lecture “Pathology of the Immunodeficiency Syndrome in animals”, 24.05.98.
• “Primates in experimental research: Primate biology and husbandry”. 2-hour
postgraduate seminar „Use of animals in experimental research“, 16.04.98.
• Participation in the postgraduate school ‚Perspectives of primatology: Integration
of genetic, neurobiological and ethological areas of primate research‘.
• Examiner at the School of Veterinary Medicine for the subjects General Pathology,
Pathological Anatomy and Histology: Preliminary examination of 32 students in
their 6th and 10th term (19.03., 20.03., 26.03., 18.09.98).
• PhD examinations at the School of Veterinary Medicine: F. Brandes, S. Müller, S.
Dag, T. Kopp, T. Kurth.
W. Kaumanns
Department of Veterinary Medicine and Primate Husbandry
• “Social behaviour of Primates.“ 1-week joint seminar with Köln University (Prof.
Dr. Dambach) and Köln Zoo, 22.-26.06.98 (summer term 1998)
Candidates for Diploma (D) and Doctorate (P)
University/Name Dissertation
School of Veterinary Medicine, Hannover
A. Didier (P)
School of Veterinary Medicine, Hannover
S. Freire Bruno (P)
School of Veterinary Medicine, Hannover
T. A. M. Janetzko (P)
Establishment of quantitative, competitive
PCR and RT-PCR to determine virus load
in intestinal biopsies of SIV-infected rhesus
monkeys
Immunohistochemical analysis of lymphocyte
subpopulations in the cecum, ileum
and the ileocecal lymph nodes in SIVinfected
rhesus monkeys (Macaca
mulatta)
Immunohistochemical analysis of intestinal
biopsies: The distribution of immunocompetent
cells in the GALT-system in rhesus
monkeys experimentally infected with SIV
Primate Report 54, July 1999 155

University/Name Dissertation
School of Veterinary Medicine, Hannover
C. Kahnt (P)
School of Veterinary Medicine, Hannover
B. Steuerwald (P)
School of Veterinary Medicine, Hannover
E. Kunz (P)
School of Veterinary Medicine, Hannover
U. Brandenburg (P)
School of Veterinary Medicine, Hannover
H. Steinmetz (P)
School of Veterinary Medicine, Hannover
T. Koch (P)
School of Veterinary Medicine, Hannover
D. Deppe (P)
School of Veterinary Medicine, Hannover
N. Kempermann (P)
University of Gießen
K. Hampe (P)
University of Tübingen
D. Stahl (P)
University of Köln
E. Krebs (P)
Universidad Complutense de Madrid
M. Esteban (P)
University of Köln
C. Schwitzer (D)
Department of Veterinary Medicine and Primate Husbandry
University of Göttingen
A. Arms (D)
University of Göttingen
P. Schmid (D)
Immunohistochemical analysis of SIVassociated
lymphomas in rhesus monkeys
Morphological analysis of the epithelium in
SIV-infected monkeys with special reference
to Helicobacter-infections
Experimental Helicobacter pylori infection
in rhesus monkeys
On the epidemiology of Helicobacteriosis
in humans: An investigation of Helicobacter-infected
cats
Alopecia in captive rhesus monkeys
Morphological analysis of rat mutants
lacking endothelium receptors, a model
for Hirschsprung-disease
The concentration of carcinoembryonic antigen
(CEA) and alphafetoprotein (AFP)
during the course of disease in the serum
of dogs with mammary tumors
The concentration of carcinoembryonic
antigen (CEA) and alphafetoprotein (AFP)
in the serum of dogs with skin tumors
Nutrition in captive primates
Social structures and feeding behaviour in
sooty mangabeys
Birth control and social behaviour in
hamadryas baboons
The effect of post-partum estrus and the
birth of an infant on the mother-child
relationship in rhesus monkeys
Feeding behaviour in red ruffed lemurs
Examination and biomechanical analysis
of locomotion in cotton-top tamarins and
squirrel monkeys
Long-term effects of demographic and
social structures on the development of
the European population of lion-tailed
macaques
156 Primate Report 54, July 1999

University/Name Dissertation
University of Nürnberg-Erlangen
K. Drueppel (D)
Tool use in mandrills
Further education
Department of Veterinary Medicine and Primate Husbandry
• F.-J. Kaup is licensed by the Lower Saxonian Chamber of Veterinarians to carry
out further training leading to a specialist veterinary qualification in Pathology.
He is also a member of the examination boards for the specialist veterinary qualifications
in pathology, wild animal care and zoo animals. In 1998, two colleagues
working at the DPZ (Dr. E-M. Kuhn, BPRC Rijkswijk) or tutored by DPZ staff (Dr.
G. Lösenbeck, Fa. Laboklin, Bad Kissingen) passed the specialist veterinary examinations
for pathology. In addition to another examination for the specialist
veterinary qualification in pathology, an examination was carried out for the specialist
qualification in wild animal care and two examinations for the specialist
veterinary qualification in meat hygiene.
• K. Wolkenhauer, a student of veterinary medicine, spent a period of practical
training in the Department of Pathology and Veterinary Medicine from 11.05.-
19.06.98. During this period she carried out an immunohistological analysis of
apoptosis in the lymph nodes of rhesus monkeys.
• Dr. J. Hauser, Working Group ‚Brain research‘ at the University of Freiburg, completed
a three-day DPZ training course on aspects of veterinary care and husbandry
of primates used in experimental research, from 01.-03.12.98.
• A one-day training seminar for high school teachers was carried out at Nürnberg
Zoo on 29.04.98. The seminar included practical exercises on the social behaviour
of primates.
• D. Wolters, training at the Institute of Veterinary Medicine, Göttingen University
for a qualification as a biological laboratory technician, worked with the project
group between 15.06.-15.08.98 as part of her professional training.
• A regular series of lectures was held on diseases of pets, histological techniques
and immunohistology. Examinations were carried out in conjunction with the local
government of Hannover at the College for Veterinary Technicians, School of
Veterinary Medicine, Hannover, during the winter term 97/98, summer term 98
and winter term 98/99.
• On behalf of the statutory veterinary office, R. Wagner, responsible for animal care
at Daun zoo, was examined on the subject of primate husbandry.
• A. Husung, Department of Primate Husbandry, is a Committee Member of the
Special Interest Group of Animal Care Staff within the Society of Experimental
Animal Care (IgTP-GV Solas).
Primate Report 54, July 1999 157

Department of Veterinary Medicine and Primate Husbandry
• The following includes people from various Institutes who have completed practicals,
training and re-training measures in the field of animal care of nonhuman
primates:
B. Oppermann (10.11.97-12.06.98), N. Rudolph (10.11.97-31.01.98), D. Rothbart
(01.08.97-31.07.99), E. Herdt (19.-30.10.98), E. Joussen (08.- 27.03.98), B. Wörner
(15.06.98), H. Lehmeier (23.11.- 05.12.98), and T. Nolte (02.-13.11.98).
158 Primate Report 54, July 1999