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Appendix - CNIC

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SCIENTIFIC REPORT ´09<br />

SELECTED PUBLICATIONS<br />

Example of high content analysis. The cells in the<br />

image were transfected with siRNA targeting inner<br />

centromere protein (INCENP) by an automated<br />

process. Note the multi-lobed nuclei. The cells were<br />

stained with Hoechst and imaged with the Opera<br />

HCS platform. The image analysis (lower panel) was<br />

performed with Acapella software, and shows cell<br />

outlines and nuclear segmentation for determination<br />

of cells with multinucleated phenotype.<br />

Aslam T, Fleck B, Patton N, Trucco M, Azegrouz H. Digital image analysis of plus disease in retinopathy of prematurity. Acta<br />

Ophthalmol (2009) 87: 368-77.<br />

Lucas D, Escudero B, Ligos JM, Segovia JC, Estrada JC, Terrados G, Blanco L, Samper E, Bernad A. Altered hematopoiesis in mice<br />

lacking DNA polymerase mu is due to inefficient double-strand break repair. PLoS Genet (2009) 5: e1000389.<br />

Marrero-Diaz R, Bravo-Cordero JJ, Megias D, Garcia MA, Bartolome RA, Teixido J, Montoya MC. Polarized MT1-MMP-CD44 interaction<br />

and CD44 cleavage during cell retraction reveal an essential role for MT1-MMP in CD44 mediated invasion. Cell Motil Cytoskeleton<br />

(2009) 66: 48-61<br />

Megias D, Marrero-Diaz R, Del Peso BM, Garcia MA, Bravo-Cordero JJ, Garcia-Grande A, Santos A, Montoya MC. Novel lambda FRET<br />

spectral confocal microscopy imaging method. Microsc Res Tech (2009) 72: 1-11<br />

Montoya MC, Piera A. Microscopía Confocal. In Técnicas en Histología y Biología Celular. Capítulo 7, p 181-208. Elsevier Masson<br />

2009<br />

76<br />

7 Technical Units

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