Appendix - CNIC
Appendix - CNIC
Appendix - CNIC
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SCIENTIFIC REPORT ´09<br />
SELECTED PUBLICATIONS<br />
Hellriegel C, Gratton E. Real-time multi-parameter spectroscopy and localization in three-dimensional single-particle tracking. R Soc<br />
Interface (2009) 6: S3-S14<br />
78<br />
7 Technical Units<br />
Left: Fluorescence Lifetime Imaging (FLIM) is one of our methods for distinguishing endogenous autofluorescent components (for example fibronectin)<br />
from multiple fluorophores in live cells. We can distinguish fluorescent proteins such as YFP, EGFP and CFP and measure FRET between them.<br />
Right: FRET pairs often cannot be inserted in the proteins of interest. In these cases the N&B approach can measure the brightness of the GFPlabeled<br />
proteins and detect their interaction, as in this example that shows ligand (ATF) induced dimerization of a GPI-anchored receptor.
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