Appendix - CNIC

SCIENTIFIC REPORT ´09
Genomics
> RESEARCH INTEREST
Head of Unit: Ana Dopazo
Support Scientists: Sergio Callejas
Alberto Benguría
Fátima Sánchez Cabo
Technician: Rebeca Álvarez
The Genomics Unit is dedicated to providing high-quality
genomic technology to the scientific community at the CNIC
and beyond. The Unit is equipped with Agilent and
Affymetrix microarray platforms, the world’s leading DNA
chip technologies, and has extensive experience in the use of
array-based technologies for genome studies. The Unit’s
expertise encompasses all the steps required for these highthroughput
approaches, including experimental design,
sample preparation and processing, and statistical data
analysis.
Major services and array-based applications include wholegenome
differential gene expression analysis (including at
the exon level) and microRNA expression analysis. The Unit’s
service portfolio has recently been expanded to incorporate
comparative genomic hybridization (CGH) arrays, a powerful
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tool for the genome-wide detection of copy number
variations. Other services include the maintenance and
management of real-time PCR instruments (one AB 7000
and two ABI 7900HT machines) and a TaqMan array
processing service. The Unit also provides user advice and
training on topics related to the Unit’s activity, particularly
experimental design and statistical data analysis for qPCR
and microarray experiments.
The Genomics Unit actively participates in one of the ongoing
CNIC clinical projects, the IM-JOVEN study. IM-JOVEN is
part of a large, multicenter case-controlled study aimed at
identifying the clinical, genetic and demographic
characteristics that determine the occurrence of myocardial
infarction in young women.