Master of Pharmacy - Nagpur University

Subject code: MPL-P10
Subject: ADVANCED PHARMACOLOGY AND PHARMACOTHERAPEUTICS
Practical: 8 hrs. /week
1. Prerequisite for Pharmacology Practicals:
In this module it is expected student should know general principles, techniques and
strategies for pharmacological screening of drugs, animal care, handling, ethical
requirements and regulations therein.
2. Basic Experimental Techniques:
1. Standard techniques collection of blood samples and feeding of animals
2. Administration of drugs by different routes in mice
3. Use of anaesthetics and cannulation of veins, arteries, trachea
3. Experiments on intact animals:
1. To study locomotor activity by using Actophotometer.
2. To evaluate analgesic activity of drug using tail flick latency test.
3. To determine the effect of carrageen induced edema in rats by using digital
Plethysmometer.
4. To study the anticonvulsant effect of Phenobarbitone against MES induced convulsio ns
in rats.
5. To determine the analgesic effect by using Eddy‟s hot plate.
6. To study effect of pentabarbitone sodium on righting reflex (hypnosis) in mice.
7. To study Anti-anxiety effect of diazepam in mice using elevated plus maze apparatus.
8. To study the Apomorphine induced compulsive behaviour (Stereotype) in mice.
9. To study the muscle relaxant property of Diazepam in mice using rotarod.
10. To study amnesic (loss of memory) effect of drug using passive avoidance step-down
task paradigm in mice.
11. To study the antidepressant effect of drug using forced swimming test apparatus.
4. Toxicity Studies:
1. Regulations and guidelines of toxicity studies
2. Method of calculation of ED50 and LD50
3. Observation of behavioral changes in animals during acute and sub acute toxicity study of
test drug.
5. Practicals using computer software’s :
In this module it is expected student should know working of software and setting of
physiologic and animal experimentation and perform at least four experiments fro m
following or others-
1. To record temperature using thermal transducer
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