elektronika electronics - Electronics Journal - Elektrotehnicki fakultet

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III. POSSIBILITIES OF TELEVISION SCANNING
OPTICAL STEREOMICROSCOPY
Television scanning optical stereomicroscopy, preserving
main idea of scanning with probe, allows to use light probe
which is free of destructive influence onto erythrocytes.
As a base for such instrumentation creation, the television
scanning optical microscope can be successfully used [1], as
its maximum working magnification is 10000x at usage of a
lens 40x and spatial resolution the tenth parts of micrometer,
allows to find out a relief of erythrocytes surface.
For analysis of erythrocytes surface architectonics it is
necessary, that the scanning optical microscope creates a 3-D
image of an object. The optical scheme of such
stereomicroscope is shown in Fig. 2.
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Fig. 2. The optical scheme of scanning stereomicroscope
The scanner 1 on projection cathode-ray tube of high
resolution with polychrome luminescence of a screen forms a
micro raster, the luminous flux from which one by a lens 2 is
transformed to a parallel pencil of rays. The interference light
filter [4] 3a reflects one of spectral components on a metallic
mirror 4a, which one routes a parallel pencil beam of light to
a microscope lens 5a, in a focus of which one there is an
investigated object 6 on a substrate 7. Diffused by the object
light, collected by a lens 5b, having reflected from a metallic
mirror 4b easily permeates the interference filter 3b on a
photo multiplier 8b, the signal from which one goes in
computer, adapted to stereo images processing and display.
The light pencil of beam with other spectral component,
passing through the interference filter 3a, is reflected from
the filter 3b and mirror 4b, falling in the lens 5b, is dispersed
by the object 6, collected by a lens 5a, reflected from a mirror
4a and through the interference filter 3a falls on a photo
multiplier 8a, the signal from which one also goes to the
computer. Thus, the interference light filters, having split a
light pencil of beam on two spectral components, lights the
object from two sides, have ensured the information on the
object, sufficient for construction 3-D image on the screen of
computer monitor. The computer image processing with
usage of a software used in a photogrammetry, allows to
compound the topographic map of investigated object relief.
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ELECTRONICS, VOL. 8, NO.1, MAY 2004.
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In Fig. 3 example of the images, experimentally obtained
with microscope action simulation by one-channel television
scanning optical microscope usage, are presented. 21x lens
was used in microscope. Fig.3a and Fig 3b show test object
as orthogonal metallic grid, covered with particles of another
metal at different magnification. Dimensions of grid cell is
30×30 µm. The erythrocytes of human’s blood (standard
fixed and dyed smear for morphological examination) are
shown in Fig. 3c. All images were not processed with
computer.
a)
b)