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STEPHAhTIE m, Department of Plant Pathology, University of Arkansas, Fayetteville, AR 72701 and KENNFM WELLS, Department of Botany, University of California ,' Davis, CA 95616. Ustilago cvnodontis intersterility groups. The consistent nature of the pairing pattern of single basidiospore isolates of Ustilago cvnodontis is confirmed. Infections of the host, Cynodon dactvlon from intra-and intercollection pairings, result in viable basidiospores. F, isolates of field collecLions and selected laboratory pairings were examined for evidence of an intersterility system epistatic to the compatibility system. The nature of the partial intersterility isolates is stable. Germination rates of F progeny from Sntracollection pairings were reduced 1 in comparison with intercollection pairings. Intercollection experiments showed variability. Pooled data showed significant differences. Growth and infection was variable, when using the same isolates on different plants, pointing to the importance of the host in the developnt of Ustilago cynodontis. J, C. OW and D. J. Mclaughlin. Dept. of Plant Biology, Univ. of Minnesota, St. Paul, MN 55108. Cytology and phylogeny of the basidiomycete XenoaloeaerioDhori. Xeno- erioDhori (Auriculariaceae sensu lato) is a heterobasidiomycete parasitic upon sedge species. It has been collected in Minnesota from Scl rDuovirem. In an effort to clarify its relationships to other auriculariaceous fungi, Xenoaloea was examined ultrastructurally and ligh: microscopically. Meiosis begins and synaptonemal complexes are formed in probasidia which are produced within the leaf clustered beneath the stomata. Metaphase I is recognized in the metabasidia which emerge through and mature outside the stomata. Basidiospores are formed which may develop ballistospores or bud as yeasts. Ultrastructural characters of nuclear division and septa1 pore structure are believed to hold particular promise in establishing a phylogeny of auricularoid fungi. Xemghzi possesses a spindle pole body (SPB) with layered discs which nucleate a well- developed array of astral microtubules from metaphase to telophase. The SPB differs structurally between meiosis I and II. The hyphal septa are perforated by multiple simple pores. J.T.~ELL~EI~, D.A. GLAWE~, M.R. TAYSEY~, and L.L. TEWS . ,Dept. Biol. Sci., Univ. Tex., El Paso, TX 79968. 'Dept. Plant Path., Univ. Ill., Urbana. IL 61801, : ~ ~ ~ Biol., t . Ind. Univ., Bloomington, IN 47405, Biol. Dept., Wisc. St. Univ., Oshkosh, WI 54911. Mycology test bank (exam file): preliminary version. The MSA Teaching Committee, with permission from the MSA Council, has compiled and edited a collection of mycology examination questions, for use by MSA members. The purpose of this test bank is to save teachers time and to improve the quality of questions available, not to provide a standardsfor assessment purposes or to suggest what should or should not be included in a curriculum. A progress report is being made at this annual meeting in order to solicit suggestions for the preliminary version of the test bank that is available for distribution. A final version will be available at the 1991 annual meeting. D. F. FAIIR. -tic Bobny and Myoology IaboliitOry, ARS, m, ElARC-WeSt, Beltsville, MD 20705 The species of -ria on The increasw in-t in the a~thraahOS2 of cornus caused by Discula sp. bas stindated the study of other frPIJi that are found on dogwoods. Eleven taw of Sevtoria have been described on Cornus. The signific~ce of bosts, spore mrphology and mnidiogenesis in the del~neation of these Sevtoria species will be discussed. T. Flynn'. O.K. Miller, J:.', and H.H. Burdsal12. Dept. of Biology, VPIBrSU, Blacksburg, Va 24060'. FPL, CFMR, P.O. Box 5130, Madison, Wi 5370j2 DNA hybridization studies on the Armillaria mellea species complex. The Armillaria mellea species complex is a cluster of morphologically similar, but reproductively isolated biological species. The extreme morphological similarity might suggest high genetic similarity, and this hypothesis was tested by comparing the genomes of56 strains representing eight North American biological species. The genomes were compared by hybridizing heterologous whole-cell DNA preparations and assaying the duplex molecules with S1 nuclease. The mating compatibility and DNA hybridization experiments compared intersterile tester strains, isolated and identified by Jim Anderson, with isolates taken from a broader geographic area which includes the states of Wa, Az, 11, and Va. The genomic similarity within a given biological species ranges from about 80-99%, and the similarity among biological species in the A. mellea complex ranges from 40-70%. The exannulate species, A. rabescens, shares about 30% similarity with the rest of the genus. These data confirm that these biological species, identified by mating compatibility, are genetically distinct entities, but identifying new strains using macroscopic compatibility assays can sometimes be misleading. Our hybrization experiments have measured similarity, and have shown variation in genetic complexity (genome size). The genetic complexity, estimated by homologous DNA reassociation rates (Cot curves), generally agree with the hybridization data. Species concepts. speciation, and alloploidy are discussed with these results in mind.
K. FCSS and J. R. GARCIA, Biology Deparbwnt, Ball State University, Muncie, IN 47306 Methylation of proteins and phospholipids during the differentiation of ME racernosus. Earlier studies with this dimorphic mold shaied that the specific activity of S-Adenosylmethionine (SAM) Synthetase, the enzyme responsible for the synthesis of SAM, increased during the aerobic germination of sporangiospores. Those studies showed that the enzyme activity remained at dormant-spore level for approximately the first 90 minutes and increased significantly after the 3 hour point, a time when the spores are rounded and swelling. The present study was undertaken in order to try and determine the reason(s) for the increase in specific activity. Studies designed to look a t total protein methylation, during spore gemination, shai an initial increase at a time when the spores are swelling and a subsequent decrease as the smllen spores begin to £om germ tubes. Fkspholipid. methylation also shms the same general pattern. An examination of individual methylated proteins revealed approximately seven proteins that are inethylated during germination. These proteins appear to be more highly methylated at a time when the swollen spores begin to synthesize gem tubes. These preliminan? results suggest that the observed increase in the specific activity of SAM Synthetase might provide additional SAM for the observed methylations. ANDREA GARGAS and JOHN W. TAYLOR Dept, of F'lant Biology, University of California, Berkeley, CA 94720. Molecular evolution and phylogeny of lichenized and non-lichenized fungi based on PCR amplified 18s rDNA sequences. This study focuses on the relationships between the ascomycetous orders Pezizales. Helotiales . Lecanorales and Caliciales . We have used the polymerase chain reaction to amplify specifically the fungal nuclear 18s rDNA from both licherized and non-lichenized fungi. Their 18s genes amplified so far vary in size from 1.8 kb to 3.2 kb, and their DNA sequences (from asymmetric amplifications) supply additional evidence for insertions in this region. Arrangements of orders within the Ascomycotina remain controversial, and DNA sequences of this conserved molecule may be used as the basis for a phylogeny which integrates both lichenized and non-lichenized fungi. In addition, these symbioses provide compact systems for studies on coevolution between the fungus and the alga. - R. 1. GESSNER and L. A. CASTLEBURY. Department of Biological Sciences, Western Illinois University, Macomb, IL 61455. Batch culture studies with Dendryphiella. Dendryphiella salina and D. arenaria are common deuteromvcetes found in coastal marine habitats. kr studied the culture conditions necessary tci grow inoculum for future fermentation studies. both species grew well on all the agar media tested and D. arenaria exhibited significantly better growth on YpSs agar. In standing broth culture, both species also grew best on YpSs. Growth occurred between 5-35 C and the optimum temperature for dry weight production was at 25-30 C. Maximum growth was achieved after 7 days in 25 ml of medium in standing 125 ml flasks. Growth was initially greater in shake flasks but by day 5 the . dry weights were not significantly greater than in standing cultures. L w. Department of Botany, Unlverslty of Tennessee, Knoxville, TN 37996- 1100. Varlatlon of matlng systens In Harasmlu&. Sixteen apecles representing seven sectlons In tlPrasmln& vere establlshed in monokaryon cultures made from fresh materlal collected from the Great Smoky and Blue Rldqe nountalns. Self-crosses revealed varlatlons in matlng system type at the sectional level. Some sectlons vere predominantly tetrapolar lbifactorlal), vhlle other sectlona vere elther predomlnantly blpolar lunlfactorlal) or furnished lnconcluslve patterns. Results of Inter-collection matlnqs vlth n. rotula, P~rrhoce~halus, B. slccua, and n. pulcherrl~es are reported, lncludlng lntercontlnental matlnqs. L. F. GRAND, R. K. JONES, AND R. D. MILHOW, Plant Pathology Department, Box 7616, North Carolina State University, Raleigh, NC 27695-7616. Colletotrichum spp. isolated from strawberries in North Carolina. In fall 1988 and spring 1989 a severe outbreak of anthracnose on strawber~y cultivar Chandler provided an opportunity to compre ane identify the three species of Colletotrichum reported m strawberry in North Carolina. Of the 280 isolates obtained, 83% were identified as C. acutatum ard 17% as _C qloeo-rioides. C. fraaariae, previously an important cause of-anthracnose in North Carolina, was not identified. C. acutatum was isolated from diseased fruit, cfowns, stolons, petioles, and leaves while s. gloeo-rioides was isolated cnly from diseased crowns. Abundant perithecia of Glomerella cingulata, the teleomorph of C. loeosporioides, were prcduce6 in culture by isolatz
Page 1 and 2: ROGER D. SOiW ISSN 0541 - 4938 Myco
Page 3 and 4: Mycological Society of America NEWS
Page 5 and 6: Abstracts of Papers and Posters S.
Page 7 and 8: isolates each Of NABS 1 and 111. tw
Page 9 and 10: J. li BHAlTACHAWJEE. Department of
Page 11 and 12: B. E. CALLAK. Pacific Forestry Cent
Page 13 and 14: of E. macrocarwum were used to inoc
Page 15: the area of the ascus apex and prol
Page 19 and 20: continents. Conidia and conidiophor
Page 21 and 22: MICHAEL T. HOLMES, EDUARDO M. VADEL
Page 23 and 24: KAY, ERIC and RYTAS VILGALYS. Botan
Page 25 and 26: sclerotiorum, pLK44.20, that when u
Page 27 and 28: and seed phosphorus concentration,
Page 29 and 30: MI and Y. Chen. Department of Biolo
Page 31 and 32: oot pieces treated with a 10% chlor
Page 33 and 34: E.A. MOMOL. J.W. KIMBROUGH, and H.C
Page 35 and 36: R. PINETTE, P. ZDROJOUY, AND S. BRO
Page 37 and 38: R.W. ROBERSON. Department of Botany
Page 39 and 40: ates of ginseng plants grown in the
Page 41 and 42: have single, deciduous spores on th
Page 43 and 44: Ascomycotina, ~asidiomycotina, and
Page 45 and 46: Michael 4. Vincent. Department of B
Page 47 and 48: C. G. Wu and J. W. Kimbrou~h. Plant
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Page 55 and 56: STEVEN L. STEPHENSON wants collecti
Page 57 and 58: RICHARD KERRIGAN needs anything on
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Page 61 and 62: TERRENCE M. HAMMILL is Acting Chair
Page 63 and 64: DON PFISTER sent along the followin
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Apply now for the James W. Sinden S
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Mycological Papers, No. 161 ASPERGI
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