s - Mycological Society of America

polyacrylamide gels. However, growth on other
nitrogen sources produces distinctive patterns
which are different from the minimal medium
pattern. In some cases (eg. with arginine and
cottonseed hydrolysate) this pattern is similar to
the nitrogen-deficient colonies; in others the
pattern is composed of selected electromorphs found
in either minimal-grown or nitrogen-deficient
colonies. The origin of the different protease
forms is not clear. However, when extracts of
colonies grown on cottonseed hydrolysate are mixed
with extracts of minimal-grown colonies, one of the
major activities fro^ the minimal extract is
destroyed. This suggests that some post-
translational modifications may be involved.
W.L LINGLE'. DJ. O'KANE~, and D. PORTER'. l~otan~
Depanment and 2~iochemistry Depanment, University of Georgia,
Athens, GA 30602
Biolumln~cc Inheritance pattcms and sexual eompaUbillty In
Pa& isolated from pine and hardwood.
Panellus srvpticus is a white-rot basidiomycete of worldwide distribution
found typically on hardwood substrates. The Nonh American variety
is bioluminescent but the nonhern European variety is not. Although
75% of single spore isolates from basidiocarps on hardwood substrates
were luminescent in culture. no single spore isolates obtained from
luminescent basidiocarps on pine (an arypical substrate) were luminescent
(as determined visually). Sexual compatibility and inheritance
of luminescence were determined for six non-luminescent monobryons
from pine crossed with themselves and crossed with 4 hardwood
monokaryons characterized as either brightly luminescent, luminescent
or non-luminescent. These crosses revealed that isolates from pine are
the same biological species as isolates from hardwood and have a bifactorial
mating system. Crosses of pine monokaryons with the
brightly luminescent monoka~on always resulted in luminescent
dikaryons, while those crossed with the luminescent monokaryon
yielded a mixture of luminescent and non-luminescent dikawons, as
did those with the non-luminescent monokaryons. Resula from
crosses of pine isolates and hardwood isolates shw that inheritance of
luminescence is more complex than earlier results (Macrae, 1942) of
crosses between the non-luminescent European variet)' and the
luminescent Nonh American variety which indicated that luminescence
was a dominant characteristic governed by one gene. Ainh and
Foerster (1%) determined that at least 2 enzymes - NAD(P)H
oxidase and luciferase - (and presumably 2 genes) were necessary for
luminescence; perhaps explaining the complex pattern observed here.
Complementation and/or additive effects of gene producu may occur.
K.F. LOBUGLIO, S.O. ROGERS, and C.J.K. WANG.
SUNY CESF, Syracuse, New York 13210.
The Cenococcum geophilum - Elaphomyces Connection.
The genus Elaphomyces (specifically species with
blackish peridia) has been proposed to be the
teleomorph, or sexual state, of C. geophilurr..
Specimens of E. anthracinus were collected at the
Huntington Wildlife Forest (SUNY CESF property) in
the New York Adirondack mountains. Thirteen
isolates of C. geophilum, representing three rDNA
(ribosomal DNA) phenotypes, were collected at this
same site two years prior to the Elaphomvces
collection. An ertempt to verify the taxonomic
connection between C. aeophilum an< Elaphomvces
was carried out by comparing their tco RI rDNA
profiles. The Eco R1 rDNA phenotypes of
-
C. geophilurn isolates from the Adirondack site
and other geographic locations, as well as DNA
isolated directly from C. geophilum mycorrhizae
adjacent to the Elaphomyces ascomata, did not
correspond with the Eco RI rDNA profile of the
Elaphomvces collected.
R. LOWEN & P. Diederich. The New York
Botanical Garden, Bronx, NY 10458 &
CUNY, 33 W. 42 St., New York, NY 10036;
5 Rue Fernand-Mertens L-2148 Luxembourg.
Two new lichenicolous spccies of
Pronectrja (Euascomycetes,
Hypocreaceae) .
A spccies of pronectria was discovered
in the UK by Hawksworth on the lichen
Xanthoria narietina. on a limestone wall.
This was the only collection of the new
taxon until Diederich collected the
species in Luxembourg on )tanthoria
parietina on a Po~ulus tree. Diederich
also collected another new species on
the lichen Thrombiun on soil in
Luxembourg. These two new lichenicolous
spccies of Pronectria will be described.
Because immersed, light colored
ascomycetes are so inconspicuous, many
more undescribed species of Pronectria
associated with their lichen hosts are
likely to be discovered.
MADOLE, GRETCHEN E, and JOHN C. C001iE. Department of
Ecology 8 Evolutionary Biology. University of Connecticut,
Avery Pt., Groton, CT. 06340.
A comparison of VAM infection on roots of Spartina patens
and Distichlus spicata in a Connecticut coastal marsh.
Studies of plants from freshwater and coastal marshes have
shown that the development of mycorrhizal infection is
affected by the underground water during the growing season.
Increase. in root colonization by vesicular-arbuscular
mycorrhizal (VAM) fungi has been noted as the water table is
lowered. During a survey of coastal marsh plants, it was
noted that Spartina patens and Distichlus spicata in high marsh
areas often showed variation in VAM infection. Root samples
from these two species were collected at several soil depths
and compared for root infection. The samples were categorized
as (1) lacking VAM fungi, (2) VAM fungi present but lacking
vesicles &/or arbuscules or, (3) VAM fungi present with
vesicles &/or arbuscules present. The results of this study
will be presented.