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Estudio de susceptibilidad in vitro

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Métodos <strong>de</strong> Referencia Americano / Europeo<br />

Formato<br />

Medio<br />

CLSI / M27-A2<br />

Levaduras 2002<br />

Macrodilución<br />

Microdilución<br />

RPMI<br />

EUCAST / E.Dis 7.1<br />

Levaduras 2003<br />

Microdilución<br />

RPMI + 2% glucosa<br />

CLSI / M38-A<br />

Filamentosos<br />

Microdilución<br />

RPMI<br />

Inóculo<br />

0,5<br />

Incubación<br />

0,5 – 2,5 x 10 3<br />

UFC/ml<br />

48h Candida spp<br />

72h Cryptococcus<br />

Lectura Visual (0,1,3,4)<br />

0,5 – 2,5 x 10 5 UFC/ml 0,4 – 5 x 10 4 UFC/ml<br />

24h Candida spp<br />

24h Rhizopus spp.<br />

48h Aspergillus spp y<br />

Fusarium spp.<br />

72h Scedosporium spp,<br />

Espectrofotométrica<br />

trica Visual (0,1,3,4)<br />

CIM /<br />

Inhibición<br />

Azoles y 5FC: 50%<br />

Anfoteric<strong>in</strong>a B: 100%<br />

Azoles y 5FC: 50%<br />

Anfoteric<strong>in</strong>a B: 100%<br />

Azoles y 5FC: 50%<br />

11<br />

Anfoteric<strong>in</strong>a B: 100%

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