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Methodes d'évaluation de la biomasse

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Centrifugation<br />

Surnageant<br />

Culot repris dans l’eau/<strong>la</strong>vage<br />

Centrifugation<br />

Surnageant<br />

Solution <strong>de</strong> Travail: DO=1<br />

2/10 4/10 6/10 8/10 1<br />

DO(600nm)<br />

Confection <strong>de</strong> <strong>la</strong> courbe Poids sec<br />

Culot repris dans un volume 20 ml<br />

Solution mère DO = 50 : x mg/ml<br />

Db: mg/ml<br />

10ml mis à sécher<br />

Les cellules sont pesées<br />

Densité bactérienne: mg/ml

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