Cellbind Screen: una nuova tecnologia di ... - Blood Transfusion

Una nuova tecnologia di affinità in microcolonnapositività da IgG, specie se deboli, sono state meglioevidenziate dalle altre tecniche in colonna.I risultati ottenuti dimostrano che Cellbind Screenha una sensibilità simile a quella degli altri sistemi incolonna, superiore a quella del LISS-AGT in provetta, euna specificità sovrapponibile a quella delle altretecniche.Bibliografia1) Axén R, Parath J, Eznback S: Chemical coupling of peptidesand proteins to polysaccharides by means of cyanogenhalides. Nature, 214, 1302, 1967.2) Björck L, Kronvall G: Purification and some properties ofstreptococcal Protein G. A novel IgG-binding agent. JImmunol, 133, 969, 1984.3) Lapierre Y, Rigal D, Adam J et al.: The gel test: a new way todetect red cell antigen-antibody reactions. Transfusion, 30,109, 1990.4) Reis KJ, Chachowski R, Cupido A et al.: Columnagglutination technology. The antiglobulin test. Transfusion,33, 639, 1993.5) Moore HC, Mollison PL: Use of a low-ionic-strength medium inmanual tests for antibody detection. Transfusion, 16, 291, 1976.6) Voak D: Validation of new technology for antibody detectionby antiglobulin tests. Transf Med, 2, 177, 1992.7) Malferrari F, Bernardoni P, Chiari E, Conte R: Una nuovaapplicazione del gel test: determinazione delle sottoclassiIgG negli anticorpi antieritrocitari. Atti del ConvegnoInterregionale per Operatori Sanitari del Servizio Trasfusionale"Simposio di Immunoematologia eritrocitaria". Genova, 13-15 novembre 1997.8) Eichler H, Böhler A, Hastka J: Micro-column affinity test andgel test: comparative study of two techniques for red cellantibody screening. Vox Sang, 77, 154,1999.9) Weisbach V, Ziener A, Zimmerman R et al.: Comparison ofthe performance of four microtube column agglutinationsystems in the detection of red cell alloantibodies. Transfusion,39, 1045, 1999.10) What is the best technique for the detection of red cellalloantibodies? International Forum, Vox Sang, 69, 292 ,1995.11) De Figueiredo M, Lima M, Morais S et al.: The gel test: someproblems and solutions. Transfus Med, 2, 115, 199212) Eichler H: The gel test: investigation on the cause and thesignificance of a positive autocontrol; in five years ofexperience with the original gel system. Diamed SatelliteSymposium, ISBT Meeting. Amsterdam, pag. 14, 1994.13) Fabijanska-Mitek J, Zupanska B, Poglod R et al.: Evaluationof the microcolumn technology for pretransfusion testing inmultiple myeloma patients. Vox Sang, 74, 31, 1988.14) Boulton FE: Increase of blood donations found positive in thedirect antiglobulin test detected by column methods: do weneed to know? Br J Biomed Sci, 53, 172, 1996.15) Moulds JM, Diekman L, Wells TD et al.: Evaluation of columntecnology for direct antiglobulin testing. Transfusion, 37(Suppl30S), S117, 1997.16) Tissot ID, Kiener C, Burnand B et al.: The direct antiglobulintest: still a place for the tube technique? Vox Sang, 77, 223,1999.17) Sokol RJ, Hewitt S, Stamps BK: Autoimmune haemolysis:an 18-years study of 865 cases referred to a regionaltransfusion centre. Br med J (Clin Res Ed), 282, 2023, 1981.383