Products at a Glance 2024

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www.pelobiotech.com Tissue Dissociation Tissue Dissociation is an extremely important early step in experiments using primary cells. This early step already determines the quality of your cell culture and finally your results. The main question is how to isolate cells in the best way and which criteria are the most important: Is it the cell viability, the biomarker profile or just the cell number? Our partner VitaCyte offers only highly purified tissue dissociation enzymes for basic research as well as for cell therapy. CIzyme TM Tissue Dissociating Enzymes Advantages of Purified Enzymes for Cell Isolation Problems with crude or enriched collagenase: Variable biochemical composition, reflects heterogeneity of the bacterial culture supernatant • Heterogenous molecular forms of collagenase Lot qualification often required Collagenases and Proteases Advantages of using purified enzymes: Uniform collagenase composition, by HPLC and specific CDA1 (CDA U/mg protein) • Purified enzymes; minimal contamination by other enzymes & endotoxin • Enzyme formulation can be modified to improve cell yield, viability, function Collagenases and Proteases Applications – Isolation of • Hepatocytes • Islet Cells • Adipose Tissue Stem Cells • Skin Fibroblast • Skin Keratinocytes • Cardiomyocytes • Glioblastoma NEW Products: Recombinant Collagenase Defined and enriched DE Collagenase products. Collagenase is blended with purified neutral protease at defined activities to provide a broad spectrum of products with distinct collagenase activity. Pic: High viable cell yields: Get consistent results with our enzyme formulations 4

U/mg DW www.pelobiotech.com PD Collagenases Changing the paradigm. VitaCyte’s purified defined (PD) collagenase products offer a new standard of versatility in optimizing cell isolations. Enriched collagenase is blended with purified neutral protease at defined activities to provide a broad spectrum of products with distinct collagenase 2,3 to neutral protease 1 activities. This design feature simplifies selection of an optimum ratio of critical enzyme activities for each cell isolation application. This manufacturing process minimizes enzyme variability enabling consistent control of cell isolations. 18000 16000 14000 12000 10000 8000 6000 4000 2000 0 Purified defined Collagenase PD 100 PD 800 CDA U/mg NP U/mg Clostripain U/mg Each PD product contains a fixed amount of protease activity (NP U 1 ), low clostripain activity 4 , and increasing amounts of collagenase activity defined by a functional collagen degradation activity (CDA U) that correlates with the biochemical form of the enyzme 2 . The target collagenase: protease activity ratios enables efficient exploration of a broad range of formulations. The three Cs of PD Collagenase CONSISTENT CONVENIENT COST EFFECTIVE • Fixed amount of purified protease • Low clostripain contamination • Removal of the majority of contaminants in crude collagenase • • Weigh out what you need or reconstitute and use entire bottle • • Rapid dissolution, no clogged filters, no need for pre-centrifugation • • Stable for 2 years at +4°C storage, 4 years at < -20°C • • Increase productivity, minimize need for lot testing • • Purchase of large quantities of “good” lots of collagenase avoided • • Easily migrate to purified enzyme products based on enzyme activity analysis of DE Collagenase www.pelobiotech.com 5