Products at a Glance 2024

www.pelobiotech.com As

www.pelobiotech.com As the stiffness of plastic culture vessels is far higher even than of bones we can imagine how artificial these cultures might be. A thin layer of a hydrogel can be helpful, but the solution will be a tuneable hydrogel. Cell Culture Reagents Insulin and Insulin-Related Products • rHu Insulin (cGMP): Recombinant human insulin used in cell culture, especially for studies involving glucose metabolism, diabetes, and insulin signaling pathways. • Bovine Insulin: Insulin derived from bovine sources, often used for similar applications as human insulin in cell culture. • Porcine Insulin: Insulin derived from porcine sources, employed in cell culture experiments related to diabetes, metabolism, and insulin signaling. • Insulin Solution, Human, Recombinant: A recombinant human insulin solution suitable for various cell culture and research applications. Cell Culture Solutions and Buffers: What about passaging? • Hanks Balanced Salt Solution without Ca2+ and Mg2+: A balanced salt solution used for cell culture applications, including the maintenance and washing of cells. • HBSS (100 ml): Hank's Balanced Salt Solution in a 100 ml format, ideal for smaller-scale cell culture experiments. • Dulbecco Phosphate-Buffered Saline, 1X (DPBS): A common buffer for cell culture applications, used for cell maintenance and experimental procedures. • PBS Buffer (1X): Phosphate-buffered saline (PBS) at a 1X concentration, widely used for cell washing and dilution in various biological assays. Chemicals and Additives • Heparin, Sodium Salt: Heparin is used as an anticoagulant in cell culture, especially when working with primary cells, stem cells, and coagulation studies. • Phenol Red (1ml) (minimum 10 vial order): Phenol red is a pH indicator often added to cell culture media to visually monitor pH changes. • Phenol Red (10ml): A larger quantity of phenol red for extended cell culture experiments. • Antibiotic Antimycotic Solution (100x): A high-concentration solution of antibiotics and antimycotics for preventing bacterial and fungal contamination in cell cultures. • Sodium Hydroxide (100 ml): Sodium hydroxide is used for adjusting pH levels in cell culture media. • L-Glutamine: An amino acid commonly added to cell culture media to promote cell growth. • 0.155 M NaCl: A saline solution used for various biological and cell culture applications. • Calcium Chloride Solution: Calcium chloride is employed in cell culture for inducing calcium-dependent processes in cells. • Lysis Buffer (5 ml): A buffer used for cell lysis and the release of cellular contents in various assays. 40

www.pelobiotech.com • Depolymerization Solution (50 ml): Used to depolymerize specific cellular components and study their properties. Reagents and Supplements • Penicillin/Streptomycin Solution (1:100): A mixture of penicillin and streptomycin, commonly used to prevent bacterial contamination in cell culture. • Bac-Off ® Antibiotic: A specific antibiotic solution used to prevent bacterial contamination in cell cultures. • Gentamycin-Amphotericin: An antimicrobial agent that includes gentamycin and amphotericin B, employed to prevent bacterial and fungal contamination. • Plating and Thawing Kit for Hepatocytes: A specialized kit designed for plating and thawing hepatocytes, commonly used in liver cell culture experiments. • Thawing Kit for Hepatocytes (suspension culture): A kit tailored for the thawing and culturing of hepatocytes in suspension. Transfection tools Cytofect Transfection Kits • Cytofect Epithelial Cell Transfection Kit: Designed for efficient transfection of epithelial cells, facilitating the introduction of foreign genetic material into these cells. • Cytofect Neuron Transfection Kit: Specialized for transfection of neurons, allowing researchers to manipulate gene expression in neuronal cells. • Cytofect Smooth Muscle Transfection Kit: Tailored for transfection of smooth muscle cells, making it possible to study gene functions in these cell types. • Cytofect Hepatocyte Transfection Kit: Optimized for transfection of hepatocytes, which is valuable for liverrelated research and gene manipulation in liver cells. • Cytofect Preadipocyte Transfection Kit: Targeted at preadipocytes, enabling efficient gene transfer into these cells, essential for studying adipocyte biology. • Cytofect Endothelial Cell Transfection Kit: Developed for endothelial cells, facilitating the introduction of genes into these cells, which is crucial for vascular biology research. • Cytofect Fibroblast Transfection Kit: Geared towards fibroblasts, allowing gene transfer to these cells commonly used in tissue engineering and wound healing studies. • Cytofect Cell Line Transfection Kit: A versatile kit suitable for transfecting various cell lines, making it a valuable tool for a broad range of research projects. • Cytofect HUVEC Transfection Kit: Targeted at human umbilical vein endothelial cells (HUVECs), supporting transfection in these specific cells. Cytofect Transfection Reagents and Enhancers • Cytofect-1 (CF1) and Cytofect-2 (CF2): Transfection reagents for use with various cell types, designed to enhance the efficiency of gene delivery. • Peptide Enhancer (PE): An enhancer that can improve the efficiency of transfection by assisting in the cellular uptake of genetic material. • Viral Enhancer (VE): Enhancer designed to improve viral transfection methods for gene delivery. Cytofect Sample Kits • Cytofect Endothelial Cell Transfection Sample Kit: A sample kit for transfecting endothelial cells to evaluate transfection efficiency and optimize transfection conditions. • Cytofect Epithelial Cell Transfection Sample Kit: Similar to the above but for epithelial cells. • Cytofect Fibroblast Transfection Sample Kit: Designed to facilitate testing of transfection conditions in fibroblasts. 41