Products at a Glance 2024

www.pelobiotech.com 3D

www.pelobiotech.com 3D Skin Model What is CellApplications’ 3D Skin Model? It is a highly physiological, three-dimensional cellular system of Human Epidermal Keratinocytes (HEK) for in vitro studies, offering an excellent cellular system to examine aspects of epithelial function and disease, particularly those related to skin biology and toxicology. Advantage: Basic science and pharmaceutical researchers use the 3D Skin Model to replace animal tests for the assessment of skin irritation due to chemicals, skin-cream- based drugs and cosmetics. Application: CAI's Skin Model can also help avoid misclassification of chemicals and skin corrosion observed in animal systems (1, 2). Other applications include phototoxicity, percutaneous absorption & penetration, wound healing and metabolism. The cells are grown and differentiated into a stratified squamous epithelium on PCF inserts with a liquid/air interface. Contact us for more information. 1) Eun & Nam, Exog Dermatol, 2:1–5 (2003) 2) York et al., Contact Dermatitis, 34:204 (1996 A. Depiction of Human Skin. B. Human Epidermal Keratinocytes (HEK) at ~80% confluency. C. Crystal Violet Staining. D. Hematoxylin & Eosin Staining of HEK differentiated into stratified squamous epithelium after 14 days. 3D Airway Model What is the 3D Airway Model? It is a highly physiological, three-dimensional cellular system of Human Bronchial Epithelial Cells (HBEpC). Application: It is used for in vitro examination of epithelial function and disease, including airway infections, tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. The cells are grown and differentiated into a pseudostriated epithelium on PCF inserts with a liquid/air interface. Fig A. Depiction of Human Airway. Fig C. Crystal Violet Staining. F i g D. Hematoxylin & Eosin Staining of HBEpC differentiated into pseudostriated epithelium after 28 days. (pic: CA) F i g B. Human Bronchial Epithelial Cell (HBEpC). The 3-D airway model is available as 12 or 24 well do-it-yourself-kit. The cells will be delivered cryopreserved. 56

www.pelobiotech.com Midifluidic Midifluidic Chambers Together with our Italian partner IVTech we offer advanced cell culture systems for 3D Cell Cultures. The Midifluidic systems (Perfusion Chambers) in the classical 24 well format can be used to perform 3D dynamic in-vitro models, following the standard protocols. IVTech is a spin-off from the University of Pisa. It was founded in January 2014 by 4 bioengineers. They have over ten years’ experience in the design and development of innovative systems for in-vitro cell culture. We provide two different systems (LiveBox 1 & 2) with transparent glass bottoms which allow live imaging during static/dynamic culture. Both systems can be connected to build up multi organ systems. Of course, we offer starter kits which include also a pump system. LiveBox 1: dynamic cell cultures. LiveBox1 is a transparent chamber designed for inter-connected dynamic cell cultures. LB1 is featured with a flow inlet and outlet for the perfusion of cell culture media. LB1 • single flow bioreactor • metabolic organs simulation LiveBox 2: for dynamic in-vitro-models of physiological barriers. LiveBox2 is transparent chamber, developed to model physiological barriers (e.g. lung and intestinal epithelium) in-vitro. LB2 is designed for inter-connected dynamic cell cultures and is equipped with two flow inlets and outlets, and a holder to house a porous membrane. • double flow bioreactor • physiological barriers simulation • equipped with a membrane LB2 LiveFlow is a compact and low weight peristaltic pump, compatible with the incubator environment. We also offer LiveFlow PRO to automate your in­vitro experiment and LivePa a pressure modulator useful to introduce pathological conditions, thus being one step closer to human reality. • compact and low weight peristaltic pump • 4 Independent fluidic circuits • 2 removable heads Why Advanced Cell Culture Systems: • Human organ environment simulation • Multi-organ models • 3D and dynamic cell cultures • Real time monitoring LiveFlow - peristaltic pump compatible with the chambers 57