Products at a Glance 2024

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www.pelobiotech.com microenvironment that mimics the conditions for metastatic cancer cell growth, researchers can gain insights into the mechanisms and factors influencing cancer spread. This information is invaluable in developing targeted therapies, assessing treatment responses, and ultimately improving the prognosis and quality of life for cancer patients. Additionally, these kits have applications in drug development, personalized medicine, and the investigation of novel therapeutic approaches to combat metastatic disease. Additional Assays Apoptosis Detection and Related Assays • Annexin V-APC/7-AAD Apoptosis Detection Kit: This assay allows for the identification of apoptotic cells by labeling them with Annexin V and 7-AAD, distinguishing between early and late apoptotic cells. • Caspase-3 Activity Colorimetric Assay Kit: It measures caspase-3 activity, a key enzyme in the apoptotic pathway. Researchers use this kit to assess apoptosis and cell death in various cell types. • TUNEL Apoptosis Assay: The TUNEL assay detects DNA fragmentation, a hallmark of apoptosis, by labeling the ends of DNA fragments. It's widely used in cancer research, drug development, and understanding programmed cell death. Cell Senescence and Related Assays • Fluorescence Senescence β-Galactosidase Activity Assay Kit: This assay detects senescent cells, which have stopped dividing and may play a role in aging and various diseases. • Chemical Senescence β-Galactosidase Activity Assay Kit: Similar to the fluorescence-based assay, this kit is used to assess cellular senescence and its impact on cell populations. 72

www.pelobiotech.com Nucleic Acid and Telomere Length Assays • Absolute and Relative Telomere Length Assay Kits: These kits are vital for measuring telomere length, which is linked to aging and various diseases, including cancer and cardiovascular conditions. • qRT-PCR Kits: These quantitative reverse transcription-polymerase chain reaction kits are used to analyze gene expression and are crucial for studying genes involved in cell cycle control and senescence. Metabolic and Redox Assays • Biopyrrin ELISA kit, Redox Assay: This assay quantifies biopyrrin, a marker for oxidative stress. It's applied to study oxidative damage in various biological systems. • Lactate Dehydrogenase (LDH) Assay: LDH is an enzyme released during cell damage. This assay helps assess cellular cytotoxicity and damage, often used in cytotoxicity studies and assessing cell health. • Creatinine, Urinary, ELISA Kit: Used to measure urinary creatinine levels, this assay aids in evaluating kidney function and diagnosing renal diseases. Cell Viability and Proliferation Assays • MTT and WST-1 Cell Viability & Proliferation Assays: These assays assess cell viability, metabolic activity, and proliferation. They are employed in drug screening, cytotoxicity testing, and assessing the effects of various compounds on cell health. TR-FRET Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a powerful and versatile molecular assay technique used in biological research and drug discovery. TR-FRET allows scientists to study molecular interactions and events within cells or biochemical reactions with high sensitivity and precision. It is particularly valuable for measuring protein-protein interactions, receptor-ligand binding, and various other biomolecular processes. TR-FRET relies on the principles of fluorescence resonance energy transfer (FRET), in which energy is transferred from one fluorophore (the donor) to another (the acceptor) when they are in close proximity, typically within 10-100 angstroms. The key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which eliminates shortlived background fluorescence and significantly enhances signal-to-noise ratios. To perform TR-FRET assays, researchers require specific reagents: • Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed 73