Products at a Glance 2024

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www.pelobiotech.com Media for Cryo-Preservation The classical way to cryopreserve primary cells is the usage of the culture medium plus 10- 20% FBS and 10% DMSO. After trypsination of adherent growing cells, neutralization of the used trypsin and centrifugation cells are resuspended in the freezing medium (e.g. Endothelial Cell Growth Medium + 10% FBS + 10% DMSO). As alternative commercially available freezing media can be used (Cryo-ROS, Cell freezing medium and others) which contain 10% DMSO. These media are available and tested for different cell types e.g. blood cells, hematopoietic stem cells, melanocytes, macrophages, microglia and neural cells. In recent years companies approached into the market who are specialized in cryo-preservation and tissue storage media. One of our partners, BioLife Solutions, Seattle, has developed a series of DMSO containing cryo media which are produced under GMP conditions and are suitable and tested for a high number of cells. CryoStor CS 2, 5 and 10 is pre-formulated with USP grade DMSO of 2, 5 or 10%. The media is based on the novel HypoThermosol formula. Cryo-Preservation Media CryoStor® CryoStor® CS10 is a state-of-the-art cryopreservation medium designed to maximize postthaw cell recovery and viability in extremely low temperatures ranging from -70°C to - 196°C. This ready-to-use solution is serum-free, animal component-free, and cGMPmanufactured, ensuring a safe and protective environment for the cryopreservation of a diverse array of sensitive cell and sample types. The formulation, containing 10% dimethyl sulfoxide (DMSO) along with other essential ingredients, is meticulously crafted with USP-grade components to minimize variability. This makes CryoStor® CS10 an ideal choice for e.g. preserving myeloma cell lines, human pluripotent stem cells, blood-derived cells. Available in various convenient formats, CryoStor® CS10 caters to the cryopreservation needs of B Cells, CHO Cells, Hematopoietic Stem and Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem and Progenitor Cells, Monocytes, Myeloma, NK Cells, Pluripotent Stem Cells, T Cells across different species, including Human, Mouse, Non-Human Primate, and Rat. Whether applied in Cord Blood Banking, Epithelial Cell Biology, Immunology, or Stem Cell Biology, CryoStor® CS10 stands out as an animal component-free, serum-free solution, ensuring the integrity and viability of preserved cells. We also offer the same with 2% and 5% DMSO. Cryo-Preservation by Vitrification Vitrification is the solidification of a liquid without crystallization and growth of ice. It is an ultrafast freezing method which is used for ES and iPS cells successfully. StemCell Keep is a DMSO-, serum-, protein- and xeno-free cryopreservation medium for ES/iPS cells by vitrification. Right: Vitrification is successful Left: Recrystallization happened vitrification failed) Procedure 1. Just add StemCell Keep to your cells after centrifugation and transfer the vial within 1 minute to liquid nitrogen. 2. Keep the vial for 1-2 minutes in liquid nitrogen and check the process. 3. Store cells in your liquid nitrogen tank. 34

www.pelobiotech.com Thawing: 1. Just take the vial out of liquid nitrogen and add 1 ml warmed medium. 2. Mix carefully and add cells to a vial with additional 9 ml medium. 3. Seed cells to your feeder plate or feeder-free plate system. Cryo-Preservation Products Cat# Product Recommended or used for Quantity FBS containing Cryo Media PB-10002-01 Cryo-ROS Cell Lines & Primary Cells 100 ml PB-10002-01-20 Cryo-ROS Cell Lines & Primary Cells 20 ml Serum-free, Protein-free, DMSO-containing Media PB-202102 CryoStor® CS 2 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle PB-205102 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle PB-205202 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bag PB-205373 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 10 mL vial PB-210102 CryoStor® CS 10 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle PB-210202 CryoStor® CS 10, Bag Adult Stem Cells, Primary Cells, Cell Lines 100 ml bag PB-210210 CryoStor® CS 10, Bag Adult Stem Cells, Primary Cells, Cell Lines 1 L bag PB-210373 CryoStor® CS 10 Adult Stem Cells, Primary Cells, Cell Lines 10 mL vial PB-210374 CryoStor®CS 10 Adult Stem Cells, Primary Cells, Cell Lines 16 mL vial PB-210473 CryoStor® CS 10, Syringe Adult Stem Cells, Primary Cells, Cell Lines 10 mL syringe PB-10003-01 Cryo-Gold CHO Cells, Hybridoma Cells, Adult Stem Cells, ES/iPS Cells, Primary Cells, Sperm, Oocyte, Embryo, Cell Lines 100 ml PB-10003-01-20 Cryo-Gold CHO Cells, Hybridoma Cells, Adult Stem Cells, ES/iPS Cells, Primary Cells, Sperm, Oocyte, Embryo, Cell Lines 20 ml PB-10004-01 Cryo-Jin iPS Cells, Human Hepatocytes, Neural Cells, Islet Cells, Oocyte 100 ml PB-10004-01-20 DMSO-free media CPL-A1 Cryo-Jin iPS Cells, Human Hepatocytes, Neural Cells, Islet Cells, Oocyte 35 20 ml NutriFreez D10 Human embryonic stem cells, iPSC, MSCs 50 mL CryoScarless Primate ES/iPS Cells, Mesenchymal Stem Cells, Cell Lines 100 ml RCHEFM002 ReproCRYO ES/iPS Cells 50 ml Vitrification medium VPL-A1 StemCell Keep ES/iPS Cells 20 ml