Products at a Glance 2024

www.pelobiotech.com

www.pelobiotech.com HydroSeev HydroSeev is a hydrogel supplement to overcome disadvantages of hydrogels like low stability and mechanical properties. HydroSeev is a biopolymer providing mechanical support to hydrogels like Alginate, Matrigel ® or Silica Extragel Matrix. Just adding 0,02% HydroSeev will improve viscosity, promotes a better porosity and mechanical stability. The distribution of cells within hydrogels is improved as well. Addition of 0.02% HydroSeev to cell cultures improves: A. Cell distribution in alginate (3.5%) hydrogel (green - HydroSeev, blue- cell clusters). B. Hydrogel viscosity is improved upon addition of HydroSeev. C. Mechanical properties of cell-seeded alginate hydrogel are significantly improved when HydroSeev is added (left). Stress-strain curve of compressed cell-seeded alginatehydrogel (right). Pic: SEEVIX Phenodrive Phenodrive, introduced by our partner Tissue Click, represents a revolutionary class of specialized substrates meticulously designed for organotypic tissue cultures and organoids. This synthetic biomaterial serves as a versatile platform for culturing and testing various cell phenotypes, manifesting as spheroids and co-culture models. Its design closely mimics tissue microenvironments, encompassing stem cell niches, epithelia, cancer masses, hepatocyte spheroids, and hypoxic conditions. Phenodrive's biomimetics seamlessly adapt to both 2D and 3D culture conditions, effortlessly accommodating commonly used plasticware. Key Features: • Composed of hyperbranched molecules presenting a range of bioligands. • Ordered presentation of cell-binding molecules at high density. • Suitable for 2D and 3D culture conditions. Cell Phenotypes: Individual ECMs are tailored for various cell phenotypes, including Stem Cells, Endothelial, Neural, Epithelial, Osteoblasts, Chondrocytes, Hepatocytes, Fibroblasts, Keratinocytes, Pancreatic beta cells, and Cancer cells. Different formulations cater to specific cell types. 54

www.pelobiotech.com Applications: • PhenoDrive-Y induces the formation of human bone marrow stromal cells (MSC) spheroids and angiogenic sprouting of endothelial cells. • PhenoDrive-I promotes the differentiation of induced pluripotent stem cells (iPSC) in brain cortical neuron networks and facilitates axonal growth in various neural cells. • PhenoDrive-R is suitable for most tissue cell phenotypes and supports the adhesion of monocytes/macrophages, mimicking their location in connective tissues. • PhenoDrive-PS induces osteoblast differentiation and is effective for MSC differentiation into osteoblasts. • PhenoDrive-U is versatile for most tissue cells and ensures adhesion through weak interactions with the cell glycocalyx, facilitating spheroid formation. • PhenoDrive-Q continuously consumes oxygen, creating localized low oxygen tension, and is recommended for cancer cell micro-masses and chondrocytes induction. User-Friendly and Flexible: • Provided as lyophilized powder. • Easily reconstituted in ethanol or aqueous media. • Compatible with various plasticware and 3D scaffolds. • Gamma- and UV-stable, maintaining optical and physicochemical properties. 3-in-1 Plate Introducing the 3-in-1 Plate, a revolutionary hydrogel insert seamlessly designed to fit within each well of a standard sixwell plate. This innovative insert offers a comprehensive platform for spheroid/organoid formation, extracellular matrix integration, and drug testing, all in one. The hydrogel comes pre-inserted within each well, with each insert featuring four quadrants capable of accommodating six spheroids/organoids per quadrant, enabling a total of 144 formations per plate. Notably, the quadrants are independent, allowing researchers to run four different experimental conditions concurrently on a single insert. The hydrogel itself is non-cell-adhesive, offering numerous benefits and ensuring disturbance-free experimentation. The 3-in-1 Plate revolutionizes spheroid/organoid culture, drug testing, and downstream analysis, all while maintaining optimal experimental consistency and reducing cell requirements. Explore the advantages of this groundbreaking platform below: • Integrated Platform: Cultivate, embed in an extracellular matrix, test drugs, and perform downstream analysis without the need to remove spheroids/organoids from the platform. • Consistent Results: Low spheroid/organoid size variance enhances experimental consistency. • Isolated Conditions: One spheroid/organoid per microwell eliminates the possibility of inter-model crosstalk, ensuring accurate and isolated experimental conditions. • Disturbance-Free Media Changes: Safely change media without the risk of aspirating or disrupting spheroids. • Reduced Cell Requirements: Low cell requirements reduce the necessity to passage cells before experimentation. • Compatibility with Imaging Techniques: The platform compatible with in situ immunostaining and imaging. • Comprehensive IHC Analysis: Researchers can perform all steps of in situ IHC analysis without removing spheroids from the platform, from dehydration to wax embedding and slicing. 55