Products at a Glance 2024

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www.pelobiotech.com PerfusionPAL What is Perfusion? Perfusion describes the rate at which blood is supplied to a given organ in the body. We believe this crucial factor can play a role in cell culture methods in order to positively impact cell survival mechanisms in vitro as well as simulate real life growth conditions for your research. Perfusion delivers oxygen to regulate metabolic activity of cells and promotes dissipation of heat. Poor perfusion may lead to cell death, and on a larger scale tissue necrosis. Our technology can be adjusted to deliver the specific perfusion rate needed for your model. What is Perfused Organ Panel? The POP system is a lab technology that combines the 3D cell culturing capabilities of the SeedEZ scaffold with the advanced oxygenation of our Blood Substitute within a compact, perfused multi-well plate, PerfusionPal. The system is ideally suited for modeling interstitial perfusion that cells in most tissues experience in vivo. Our PerfusionPal plate is easily perfused with a single tube that is attached to the base of the plate. The tube is flexible and has a durable metal sheath that you can use to route the tubing between the gasket and the door of the incubator. At the other end of the tubing is a syringe pump, with room for 2 syringes to operate 2 PerfusionPal plates at once. The perfusion rate is set by the user and can be easily paused for alterations, or stopped for media changes. Pumping of this blood substitute acts as a "liquid piston" that introduces perfusion uniformly along the plate, without the need for separate pumps and tubing for each culture. pics. Lena Biosciences Applications include: • Small molecule drug testing: drug metabolism, on-target efficacy & off-target toxicity • Large molecule biopharmaceutical testing: interstitial delivery mimicking in vivo conditions, on-target efficacy/ off-target toxicity of antibody-drug-conjugates & multi-specific antibodies • Cancer research: simulate advanced, vascularized tumors with heterogeneous cell populations, extracellular matrix, & intratumor supply of drugs to more closely mimic a patient’s tumor at the time of treatment How does it work? Step 1: Add Blood Substitute to the bottom of the PerfusionPal tray Step 2: Insert middle component containing wells, and add media to each well Step 3: Seed cells into SeedEZ scaffold and place scaffolds into wells. Set perfusion rate on pump and begin perfusion 60

www.pelobiotech.com Benefits of using PerfusionPAL : • Superior oxygenation and gas exchange • Physiologically relevant interstitial perfusion • Long-term culturing, testing and analysis • In-well assaying (supernatants can be transferred to another plate for reads) • Time-course analyses by sampling secretome and metabolome from wells, using ordinary micropipette • Customizable perfusion rates • Reliable perfusion (there are no bubbles and no microchannels to clog) • Statistically independent wells • Zero dead volume in fluidics • Sterile and ready to use • Blood Substitute can be reused, and maintains purity • Blood Substitute is inert and immiscible with media, reagents, drugs etc. • Offers more precision and control compared to animal models GradientEZ GradientEZ is a qualitative cell migration and invasion assay that enables cells to migrate in three dimensions as they do in living tissues. It is straightforward to use and works like a piece of paper. Simply, pipet cells in the center and add test agents to petals or lobes to model physiologically relevant, gradient-dependent pathological conditions in 3D. Alternatively, add invasive cancer cells to the center and normal cells to lobes. Fluorescently labeled cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell migration studies using fluorescence microscopy. Suitable for both adherent and suspension cells. How does it work? Method 1: Migrating or invading cells, and different cell populations or test compounds may be added to the center and 6 petals or lobes Method 2: To test metastatic potential of cancer cells in vitro, add invasive cells to the center and normal cells (e.g. brain, lung, liver, bone, adrenal, etc.) to lobes and track invasion to begin to elucidate where they might metastasize in vivo. Benefits of using GradientEZ: • Closely mimics physiologically relevant in vivo conditions • Fits in any Petri dish, 6-well to 12-well plates & 6-well inserts • Test modulators of cell motility or invasion, in parallel, in one assay • Up to six different concentrations of the same agent can be tested in one well Applications: • Cell migration assays, cell invasion assays, chemo-invasion assays, chemotaxis assays 61