Análise química e da atividade antimicrobiana dos óleos ... - UFRJ

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AbstractMARTINI, Marcia Guimarães. Chemical Composition and Antimicrobial Activityof the Essential Oils from Ocimum selloi Benth, Hesperozygis myrtoides (A.St.-Hil.) Epling (Lamiaceae). Dissertation (Master in Pharmaceutical Sciences).Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro,2011.The family Lamiaceae has approximately 220 classes and about 3,500 to 4,000species. Species of this family are known to be very aromatic and therefore are usedin cooking. Ocimum selloi Benth is a Brazilian native plant, used in traditionalmedicine and sold in open-air markets in the State of Rio de Janeiro. Hesperozygismyrtoides (A. St.-Hil.) Epling is a very aromatic shrub found in the State of MinasGerais, which grows at altitudes of 1800 meters. Mentha pulegium L. originates in theMediterranean region and parts of Asia, with a pleasant aroma of "mint" and animportant chemical polymorphism. The objective of this study was to analyze thevolatile constituents from different individuals of O. selloi, H.myrtoides and M. pulegium volatile composition to correlate a volatile compositionwith possible chemotypes, as well as evaluating the antimicrobial activity of essentialoils and perform accelerated stability study. O. selloi was purchased from open-airmarkets, while the M. pulegium was purchased from an herbal producer of the city ofTombos/ MG. The H. myrtoides was collected in the top of mountains around the cityof Aiuruoca/ MG. The essential oils of these species were extracted byhydrodistillation in modified Clevenger apparatus and analyzed by GC-FID and GC-MS. The antimicrobial tests were made by “drop test” and the MIC against strains ofGram positive and negative bacteria. MIC was also done for clinical strains. Theaccelerated stability test was conducted in a climatic chamber, according to theANVISA protocols (2005). The Metilchavicol substance was identified as thepredominant in all the oils of O. selloi, ranging from 92.7% to 97.6%. The majorconstituents of the essential oil of H. Myrtoides were identified as pulegone (44.4%)
and isomenthone (32.7%). The essential oil of M. pulegium showed isomenthone(33.6%) and pulegone (32.8%) as the predominant constituents. All essential oilsshowed antimicrobial activity for the microorganisms tested, and the clinical strainsof Candida albicans and glabrata were the most susceptible. Combination indifferent proportions of the essential oils of O. selloi and H. myrtoides were tested fortheir antimicrobial activity against C. albicans, however, there was no change inactivity at the concentrations tested. The result of the accelerated stability testshowed that the essential oils of O. selloi and H. myrtoides were stable, even thoughtheir color changed, as the major constituents of the essential oilof O. selloi (metilchavicol) and H. myrtoides (pulegone and isomenthone) had littlevariation in content during the six-month study. Therefore, the essential oils of thesespecies may be employed in pharmaceutical formulations aimed to treat infectionscaused by Candida.Key-words: Lamiaceae, Ocimum, Hesperozygis, Mentha, essential oils, antimicrobial activity
Page 1 and 2: UNIVERSIDADE FEDERAL DO RIO DE JANE
Page 3 and 4: FICHA CATALOGRÁFICAM386aMartini, M
Page 5 and 6: Dedico este trabalho aos meus pais
Page 7 and 8: Aos meus colegas de laboratórios:
Page 9: ResumoMARTINI, Marcia Guimarães. A
Page 13 and 14: 3.3.2 Determinação da concentraç
Page 15 and 16: Figura 20 - Cromatograma do óleo e
Page 17 and 18: Lista de Siglas e AbreviaturasANVIS
Page 19 and 20: Cita-se como exemplo o gênero Lava
Page 21 and 22: por leigos como condimentos em culi
Page 23 and 24: Tabela 2 - Comparação da composi
Page 25 and 26: Estudos da composição volátil de
Page 27 and 28: essenciais ricos em pulegona (Fig.
Page 29 and 30: 1.3 Óleos essenciaisÓleos essenci
Page 31 and 32: Os efeitos biológicos dos óleos e
Page 33 and 34: longo da história em pomadas e bá
Page 35 and 36: Como exemplos de espécies ricas em
Page 37 and 38: (50 -70 %), da boca (30 - 50 %), da
Page 39 and 40: 2 Objetivos2.1 Objetivo geralAnalis
Page 41 and 42: foram quantificados e reunidos para
Page 43 and 44: 3.3.2 Determinação da concentraç
Page 45 and 46: 4 Resultados e Discussão4.1Teor e
Page 47 and 48: metilchavicolFigura 18 - Cromatogra
Page 49 and 50: submetidas à hidrodestilação, pr
Page 51 and 52: Tabela 7 - Comparação da composi
Page 53 and 54: Tabela 9 - Substâncias identificad
Page 55 and 56: 4.1.3 M. pulegium - O óleo essenci
Page 57 and 58: Figura 20 - Cromatograma do óleo e
Page 59 and 60: 4.2 Atividade antimicrobiana dos ó
Page 61 and 62:
Tabela 15- CIM dos óleos essenciai
Page 63 and 64:
4.3 Teste de estabilidade acelerado
Page 65 and 66:
Tabela 17 - Tabela das análises do
Page 67 and 68:
MetilchavicolFigura 22- Cromatogram
Page 69 and 70:
4.3.2 H. myrtoides: análises croma
Page 71 and 72:
Teor %4540353025201510501 2 3 4 5 6
Page 73 and 74:
Figura 26- Cromatograma da 2ª Aná
Page 75 and 76:
5 ConclusõesA partir das análises
Page 77 and 78:
6 Referências bibliográficasADAMS
Page 79 and 80:
DUARTE, M.C.T.; FIGUEIRA, G.M.; SAR
Page 81 and 82:
MARTINS, R.E, CASALI, W.D.V., BARBO
Page 83:
SMITH,R.L., COHEN, S.M., DOULL, J.,
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Análise química e da atividade antimicrobiana dos óleos ... - UFRJ

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