Том 2 №2 - Общество Ð‘Ð¸Ð¾Ñ‚ÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¾Ð² России им. Ю.А. Овчинникова

Вестник биотехнологии, 2006, 2, № 215. Rouge P., Cambillau C., Bourne Y. The three-dimensionalstructure of legume lectins / Lectin Reviews. – St. Louis,Sigma Chem. Comp., 1991. – Vol. 1. – P. 143–159.16 Sharon N. Lectin-carbohydrate complexes of plants andanimals: an atomic view // Trends Biochem. Sci. – 1993.– Vol. 18. – P. 221–226.17. Olson E.R., Sadowsky M. J., Verma D.P.S. Identification ofgenes involved in the Rhizobium-legume symbiosis by Mu-di(Kan, Lac)-generated transcription fusions // Biotechnology.– 1985. – Vol. 3. – P. 143–149.18. Bhuvaneswari T.V., Bauer W.D. Role of lectins in plantmicroorganisminteractions // Plant Physiol. – 1978. – Vol.62. – P. 71–74.19. Lodeiro A.R., Lopez-Garcia S.L., Vazquez T.E.E.,Favelukes G. Stimulation of adhesiveness, infectivity, andcompetitiveness for nodulation of Bradyrhizobium japonicumby its pretreatment with soybean seed lectin // FEMSMicrobiol. Lett. – 2000. – Vol. 188. – P. 177–184.20. Bhuvaneswari T.V., Mills K.K., Crist D.K., Evans W.R.,Bauer W.D. Effect of culture age on symbiotic infectivity ofBradyrhizobium japonicum // J. Bacteriol. – 1983. – Vol.153. – P. 443–451.21. Joshi P.A., Caetano-Anolles G., Graham E.T., GresshoffP.M. Ontogeny and ultrastructure of spontaneous nodules inalfalfa (Medicago sativa) // Protoplasma. – 1991. – Vol.162. – P. 1–11.22. Brill L.M., Fujishige N.A., Hackworth C.A., Hirsch A.M.Expression of MsLEC1 transgenes in alfalfa plants causessymbiotic abnormalities // Mol. Plant Microbe Interact.– 2004. – Vol. 17. – P. 16–26.23. Федоров С.Н., Симаров Б.В. Получение мутантов с измененнымисимбиотическими свойствами у Rhizobium melilotiпод действием УФ-лучей // С.-х. биология. – 1987. – Т.9. – С. 44–49.THE SYNTHESIZED IN E. COLI PEA LECTIN is CAPABLETO INITIATE a NODULATION OF ALFALFA PRETREATEDBY R. LEGUMINOSARUM BV. VICIAEI.I. GUBAYDULLIN, Al.Kh. BAYMIEV, An.Kh. BAYMIEV, A.V. CHEMERIS, V.A. VAKHITOVInstitute of Biochemistry and Genetics, Ufa Scientific Center of Russian Academy of SciencesThe full-size Pisum sativum lectin gene was cloned. The pea lectin, synthesized as a single polypeptide chain uncleaved prolectinin E. coli has shown functionality; under pre-treatment by this lectin Rhizobium leguminosarum bv. viciae we have found giantnoninfection multilobed nodules not observed in control variants in roots of alfalfa. Thereby, this prolectin produced in the expressionprokaryotic system saves not only sugar binding properties, as well as also some their own physiological characteristics, causing specificnodulation reactions.Keywords: legume-rhizobial symbiosis, lectins, Pisum sativum.16