Bacher - LC/MS

Applikation 2: Analyse von ETU in Gemüseproben mit
LC/MS/MS Bestimmung
Beispiel für die Nachweisstärke der Triple-Quad LC/MS/MS
Sample Name: "K793-89" Sample ID: "" File: "P793etu#076.wiff"
Peak Name: "ETU" Mass(es): "103.0/59.8 amu"
Comment: "Cal 5.0 ng/mL" Annotation: "P793etu-05d.dam"
Sample Index: 1
Sample Type:
Concentration:
Standard
5.00 ng/mL
1200
Calculated Conc: 5.19
Acq. Date: 10-Mar-05
Acq. Time: 22:45:04
ng/mL 1150
1100
Modified: Yes
Bunching Factor: 2
Noise Threshold: 11.32 cps
Area Threshold: 56.58 cps
Num. Smooths: 2
RT Window: 30.0 sec
Expected RT: 3.87 min
Sep. Width: 0.20
Sep. Height: 0.01
Exp. Peak Ratio: 5.00
Exp. Adj. Ratio: 4.00
Exp. Val. Ratio: 3.00
Use Relative RT: No
Kalibrierlösung ETU, 5 ng/mL �0.004 mg/kg LOD (20 % LOQ)
Int. Type: Manual
Retention Time: 3.85 min
Area: 1.36e+004 counts
Height: 1.10e+003 cps
Start Time: 3.67 min
End Time: 4.25 min
In te n s ity , c p s
1050
1000
950
900
850
800
750
700
650
600
550
500
450
400
350
300
250
200
0.71
150 0.28 0.95 1.70
1.98
100
3.26
5.98
50
0
3.85
1 2 3 4 5 6 7
Time, min
Übergang 103 > 60 m/z
7.71
Sample Name: "K793-89" Sample ID: "" File: "P793etu#076.wiff"
Peak Name: "ETU 2. Übergang" Mass(es): "103.0/44.0 amu"
Comment: "Cal 5.0 ng/mL" Annotation: "P793etu-05d.dam"
Sample Index: 1
Sample Type: Standard
Concentration:
Calculated Conc:
5.00
5.19
ng/mL
ng/mL
500
Acq. Date: 10-Mar-05
480
Acq. Time: 22:45:04
460
Modified:
Bunching Factor:
Yes
2
440
Noise Threshold: 10.91 cps
420
Area Threshold:
Num. Smooths:
54.57
2
cps
400
RT Window:
Expected RT:
Sep. Width:
30.0
3.88
0.20
sec
min
380
360
Sep. Height:
Exp. Peak Ratio:
0.01
5.00
340
Exp. Adj. Ratio: 4.00
320
Exp. Val. Ratio:
Use Relative RT:
3.00
No
300
Int. Type: Manual
Retention Time: 3.88 min
Area: 4.45e+003 counts
Height: 3.56e+002 cps
Start Time: 3.66 min
End Time: 4.22 min
In te n sity , c p s
280
260
240
0.28
220
200
180
0.96
2.05
2.99
2.66
3.05
160
140
120
100
80
60
40
20
0
3.88
4.68
5.26
6.19 6.82
1 2 3 4 5 6 7
Time, min
Übergang 103 > 44 m/z
7.55