ARTIcLE ima funGuS
doi:10.5598/imafungus.2012.03.01.01 INtroductIoN Tibouchina granulosa (Melastomataceae), the Brazilian glorytree, is a fast growing tree native that occurs in the Atlantic Forest of Brazil. It is a common and important component of the native flora, particularly in secondary forests. It is also a highly prized ornamental, which is widely used in gardens and parks because of its spectacular violet or pink blossoms that each year appear prior and during Easter. The period of lent (quaresma, in Portuguese) is an important period in the catholic tradition, from which the Brazilian common name for this tree, “quaresmeira” is derived (Lorenzi 2002). Usually there is a high demand for young T. granulosa plants in the garden nursery market in Brazil, particularly for the variety that produces pink flowers. Nevertheless, diseases are known to be a limiting factor in nursery production of T. granulosa. However, very little is known about the diseases affecting this tree. The record of Chrysoporthe cubensis (syn.: Cryphonectria cubensis) on T. granulosa in Brazil by Seixas et al. (2004) is the sole record of a fungal pathogen on this host in the Brazilian database (http://pragawall.cenargen. embrapa.br/aiqweb/michtml/fichahp.asp?id=1912)–and there are only three records of fungi on this host in the USDA fungal database (Farr & Rossman 2010). This is somewhat surprising for such a common plant in the neotropics, and possibly reflects the limited existing knowledge about plant pathogenic fungi occurring on wild plants in this region. Here we clarify the identity of the fungus associated with a severe foliage blight (often evolving into a form of dieback) of quaresmeira. This is one of the most widespread © 2012 International Mycological Association You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights. volume 3 · no. 1 and damaging diseases affecting T. granulosa in the field, in gardens, and also in nurseries. MAterIAl ANd Methods <strong>IMA</strong> FuNgus · voluMe 3 · No 1: 1–7 Pilidiella tibouchinae sp. nov. associated with foliage blight of Tibouchina granulosa (quaresmeira) in Brazil Bruno E.C. Miranda 1 , Robert W. Barreto 1 , Pedro W. Crous 2 , and Johannes Z. Groenewald 2 1Universidade Federal de Viçosa, Departamento de Fitopatologia, 36570-000, Viçosa, MG, Brazil; corresponding author e-mail: Robert W. Barreto, rbarreto@ufv.br 2CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands Abstract: Tibouchina granulosa (Melastomataceae), Brazilian glorytree (Brazilian common name – quaresmeira), a common tree of the Atlantic Forest of Brazil, is widely used as an ornamental for its violet or pink blossoms. Little is known about fungal diseases affecting this species, although these represent a known limitation for its cultivation in nurseries. Among these there is a foliage blight that occurs in combination with distortion of branch apices and die-back. A consistent association of a species of Pilidiella with the diseased t<strong>issue</strong>s was observed. The fungus was isolated in pure culture and based on its morphology and DNA phylogeny, we conclude that it represents a new species, for which the name Pilidiella tibouchinae is introduced. Article info: Submitted: 9 January 2012; Accepted: 29 February 2012; Published: 5 April 2012. Key words: Coniella Diaporthales ITS LSU Sordariomycetes systematics Isolates Samples of young abnormal branches of Tibouchina granulosa bearing diseased leaves were collected at two localities in Brazil (states of Rio de Janeiro and Minas Gerais), dried in a plant press and brought to the laboratory for further examination. Representative specimens of the fungus were deposited in the herbarium at the Universidade Federal de Viçosa (VIC). Pure cultures were obtained by transfer of conidia, using a sterile fine-pointed needle, from lesions onto plates containing VBA (vegetable broth-agar) as described in Pereira et al. (2003). Pure cultures are deposited in the fungal culture collection at the Universidade Federal de Viçosa and also at the CBS-KNAW Fungal Biodiversity Centre (CBS) in Utrecht, The Netherlands. Representative voucher specimens are deposited in VIC and at CBS. DNA isolation, amplification and analyses Genomic DNA was isolated from fungal mycelium grown on MEA, using the UltraCleanTM Microbial DNA Isolation Kit (MoBio Laboratories, Inc., Solana Beach, CA, USA) according to the manufacturer’s protocols. The primers V9G (de Hoog & Gerrits van den Ende 1998) and LR5 (Vilgalys & Hester 1990) were used to amplify part of the nuclear rDNA operon spanning the 3’ end of the 18S rRNA ARTIcLE 1
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