Dye Separation - Leica Microsystems

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18 Confocal Application Letter A dialog box will open automatically. The measured emission spectrum of the fl uorophore is displayed and can be saved in the spectra database. ➅ ➃ ➄ ➆
4. Fill in the fi elds ➃ accordingly. By clicking on Save ➄ the spectrum is saved under User in the Table of spectra ➅. 5. Press X ➆ to go back to the Spectral Dye Separation dialog. The saved spectrum is now available in the list under User ➇. You may continue as described under paragraph A. ➇ Part of the image gallery of a lambda series of Drosophila melanogaster stained with Alexa 488, Alexa 546, Alexa 568 and TOTO-3. ➅ Dye Separation Confocal Application Letter 19
Page 1 and 2: CONFOCAL APPLICATION LETTER reSOLUT
Page 3 and 4: Fig. 2a: Emission spectrum of the g
Page 5 and 6: 1.1 Channel Dye Separation 1.2 Spec
Page 7 and 8: 1.4 Automatic Dye Separation: Weak
Page 9 and 10: 2. Dye Separation: Choosing the Rig
Page 11 and 12: The histogram visualizes the color
Page 13 and 14: 2. Place the crosshair in the viewe
Page 15 and 16: Separation of two fl uorophores Ori
Page 17: 2. In the fi eld Measurement Area
Page 21 and 22: ➀ 1. Select the Manual ➀ method
Page 23 and 24: 3.4 Automatic Dye Separation: Weak

Dye Separation - Leica Microsystems

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