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Dye Separation - Leica Microsystems

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20 Confocal Application Letter<br />

Fluorescence signals are separated after processing the spectral data with the Spectral <strong>Dye</strong> <strong>Separation</strong> tool.<br />

Courtesy of Dr. Ralf Pfl anz, MPI Biophysical Chemistry, Göttingen<br />

3.3 Manual <strong>Dye</strong> <strong>Separation</strong><br />

Clicking on Automatic opens a dialog box, where you can choose the settings for either the Manual<br />

<strong>Dye</strong> <strong>Separation</strong> or the Automatic <strong>Dye</strong> <strong>Separation</strong> methods. Choosing Manual opens a new dialog<br />

window.

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