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females showed no significant differences
between secretin- and pancreozymin-treated
animals. Periods 3 (six to 10 hours) and 4 (10 to
20 hours) exhibited no significant differences
between the two treatments for either sex.
Protein concentration was two to three-fold
greater in pancreozymin-treated animals than in
those treated with secretin in periods 1 and 2 in
both sexes. Secretin-treated hamsters remained
relatively constant in terms of protein
concentration in secretions throughout the entire
collection period. Large differences were also
seen in sodium concentration between secretin-
and pancreozymin-treated animals, with
pancreozymin treatment eliciting about two and a
half times as much sodium. K + concentration was
40 to 50% lower in the case of secretin
stimulation, as was Ca ++ concentration and Mg ++
concentration. Chloride and bicarbonate were
similar in the case of treatment by either hormone,
whereas sulfate values were 60 to 90% of those
for pancreozymin. Phosphate, on the other hand,
was two times the value of pancreozymin-
stimulated juice in the case of secretin stimulation.
Electrophoresis showed a pattern similar to that in
untreated animals (see above). Secretin
depressed DNA synthesis but had little effect on
RNA synthesis and slightly depressed protein
synthesis in the entire pancreas. Pancreozymin
appeared to have no effect upon DNA synthesis
but seemed to stimulate RNA synthesis.
Pancreozymin did not affect protein synthesis 90, 91 .
5c. Pattern of Pancreatic Secretions in
Aging Hamsters
Pancreatic secretions were collected at eightweek
intervals from eight weeks to 28 weeks of
age and analyzed for all of the aforementioned
parameters, exclusive of DNA, RNA, and protein
90,91
synthesis . The pH remained relatively
constant in all of the collections. The flow rate was
either similar to eight-week values or lower in all
but three instances. Twelve-week-old male
hamsters had a flow rate higher than that that of
eight week-old at the 0 to 3 hour collection period.
The 28-week-old male hamster had higher than
28
baseline flow rates at six to 10 hours and 10 to 20
hours.
i. Protein concentration was at least five times
higher than the eight-week baseline values in
12-, 20- and 28-week-old animals.
ii. Na+ concentration was higher by approximately
2.5-fold in hamsters at the 12th
week. At 20 and 28 weeks, the Na+ concentration
was similar to the baseline.
iii. K+ concentration was lower than the baseline
in the collections from the12-week-old female
controls during all collection periods. In the
corresponding male controls, K+ was higher in
the collection at 0 to 3 hours, similar in the
three to six hours and six to10 hour periods
and slightly lower in the 10 to 20 hour
collection. The K + concentration was
significantly lower in all collection periods in
both sexes with values around one-fourth
those of the baseline, in 20-week-old
hamsters. Twenty-eight-week-old hamsters
showed values for K + which were 1/10 those
of the baseline values throughout.
iv. Ca++ values were also lower during all
collection periods in 12-, 20-, and 28-week-old
hamsters with 12-week values being one-half
that of the baseline, and with 20- and 28-week
values being about one-fifth of baseline
amounts.
v. The tendency was for magnesium values to
become lower from 12 to 28 weeks as well.
vi. Bicarbonate values were lower at 12 weeks of
age, being one-fourth those of baseline values
for both sexes through all collection periods.
During the 20th week, hamsters exhibited
amounts of bicarbonate which were about
one-sixth those of the baseline, while the
amount of bicarbonate increased to one-third
of baseline values in all 28-week collections.
vii. Concentrations of chloride were similar to
those of the baseline at 12 weeks, while at 20
weeks all collections for both sexes were
about 1.5-fold higher [except in period 1 (0 to
3 hours) in which the values were almost