DiSA_ttivo © DiSA - Università degli studi di Foggia - Agraria ...

AlicyclobAcillus Acidoterrestris:
un alterante emergente
compounds (10–500 ppm) were solved in malt extract broth, inoculated separately
(with 10 3 spores ml) of each strain; the samples were incubated at 44°C and the
outgrowth of spores was evaluated every day by measuring the absorbance of
the medium at 420 nm; inoculated samples without active compounds were used
as controls. The results pointed out that limonene was not effective in inhibiting
the outgrowth of A. acidoterrestris spores; 100 ppm of cinnamaldehyde or sodium
benzoate slowed the spore germination, whereas 500 ppm of eugenol inhibited
the growth of microbial targets for 13 days. Strain c8 was more resistant than
isolate c4 and cinnamaldehyde was the most effective compound in inhibiting
the germination of A. acidoterrestris spores.
2 Alicyclobacillus
acidoterrestris: new methods for inhibiting spore
germination. Bevilacqua, A., Sinigaglia, M., Corbo, M.R. International Journal
of Food Microbiology, 125, 103-110 (2008). Impact Factor: 2,581.
For a long period the thermal processing has been considered as the only
way to reduce the initial spore number of Alicyclobacillus acidoterrestris
and prevent the spoilage of acidic beverage. New methods, however, were
proposed by the literature to control spore germination both in laboratory
media and in real systems. After a brief introduction on the impact of
A. acidoterrestris in food microbiology and a description of enumeration
methods and heat processing applied by the juices manufactures, a review
of innovative approaches to inhibit and/or control spore germination is
proposed. In particular, this paper focuses on two different topics; the 1 st is the
use of some natural compounds (monolaurin, lysozyme, nisin and essential
oils) or some chemicals, conventional (like sodium-benzoate, organic acids,
surfactants and chlorine dioxide) or not conventional (chlorine dioxide as
gas). The 2 nd topic is a description of some innovative methods to reduce
the initial spore number (high hydrostatic and homogenisation pressures,
radiation and microwaves).
34
DiSA_ttivo