Miniature Sensors for Biological Warfare Agents using Fatty Acid ...

3.5. Chemicals ...........................................................................45
3.5.1. Fatty acids and fatty acid methyl esters..............................45
3.5.2. Freeze-dried Bacteria .........................................................45
3.5.3. Alternative Methylating Agents ...........................................47
4. Results and Discussion...................................................................................48
4.1. Micropyrolysis .....................................................................48
4.1.1. Device characteristics.........................................................48
4.1.2. Test fixtures ........................................................................50
4.1.3. Sample vaporization ...........................................................51
4.1.4. Micropyrolysis / methylation of chemicals ...........................54
4.1.5. Micropyrolysis / methylation of whole cells / spores............59
4.1.6. Alternative Reagents...........................................................61
4.1.7. Colorado School of Mines micropyrolysis ...........................62
4.1.8. Summary ............................................................................64
4.2. Microfabricated GC Column................................................65
4.2.1. Isothermal ...........................................................................66
4.2.2. Temperature ramped ..........................................................67
4.2.3. Summary ............................................................................70
4.3. Surface Acoustic Wave Detection.......................................70
4.3.1. FAMEs................................................................................70
4.3.2. Dipicolinic acid ....................................................................73
4.3.3. Summary ............................................................................73
4.4. Issues to Resolve ...............................................................73
5. Conclusions ....................................................................................................74
6. References......................................................................................................75
7. Appendix A: Reference mass spectra.............................................................81
7.1. Dipicolinic- and picolinic-related compounds. .....................81
7.2. Saturated fatty acid methyl esters.......................................82
7.3. Unsaturated fatty acid methyl esters...................................86
7.4. FAME fragment ions ...........................................................88
8. Appendix B: Chemical reference and physical data........................................89
9. Appendix C: Commercial FAME chromatograms............................................93
10. Appendix D: Canola oil Reference Information .............................................96
11. Appendix E: Packed column FAMEs ............................................................97
12. Appendix F: Commercial Aerosol Collectors/Samplers.................................98
13. Distribution ..................................................................................................102
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