CONTRIBUTION TO CINCHONA ALKALOIDS CHEMISTRY

Ph.D. Thesis Abstract Contribution to cinchona alkaloids chemistry
a – area determined by chromatography of the analyte solution
Table 2.21 presents the results of testing the inearity for the HPLC method .
Tabel 2.21. Results of the linearity tesing
Parametru Valori
Concentration (μg/ml) 10 -50
Regression coefficient(R 2 ) 0,9998
Slope 886725
.
In the range of concentration 0.01 mg / ml - 0.05 mg / ml there is a linearity between peak
area of QCI-ASA sample and the sample concentration , with a correlation coefficient R 2 =
0,9998 ≥ 0,99. A minimum correlation coefficient of 0.99 is the minimum upheld.
2.4.2.3. . Determining the limit of detection and limit of quantification
The limit of detection is equal to the concentration for which the
signal analyte /noise is equal to at least 3:1.
Detection limit can be calculated based on standard deviation of the response and slope
of linearity_line [ICH]
LD = 3.3 α / P LQ =10 α / P
Where: α - the standard deviation of blank sample
P – slope
To determine the standard deviation of the blank sample, a sample is injected three times .
It is prepared similarly to sample preparation and analysis without introducing the analyte. Inject
3 three times this solution and record the noise peak area retention time corresponding to
the analyte peak. The results are presented in Table 2.22.
Tabel 2.22. Blank analysis results
Nr.
Probă
Timp de
retenție
(min)
Arie pic
(mV·s)
40
Thus it was determined:
α = 12.58 mV • s
P = 8867525
Applying the calculation of
detection limit and quantification
limit we obtain: