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146 oSBAKKEN ET AL .
sereral sets of experiments were performed to determine the etfects of saturation .
Delay times (TR time) of 4, 8 . 12 . 16. and 20 s were used . No change in signal/noise
occurred for ATP or P, with a TR time of 4 s or greater . The PCr siplal increased
progressively up to a TR time of 16 s(16% incpease from a 4 f TR time), with no
funher increase in PCr signal with longer TR times . Since only one p:ak, the PCr
peak, would experience saturation effecu and because we were osing taYeo data and
not pertonning absolute quantification, we chose not to apply a correction /atctor for
saturation to our data .
The Pmblinn uf glotd ?.3J)ipposphughar~dre
Samples otblood from three cats were taken and placed immediately in NMR tubes
and studied at room remperature with 1aP NMR using a 12 .4-T magnet to determine
the quantity of 2 .3-diphosphoglrceric acid in the btood which might contaminate the
heart spectm under in rnn co:n3itions . Spectra wers obtained using melhylene diphosphonic
acid (Meth Phos) as a standard. as wasdodie in trro. Inaddition. NaH2P0s
was used as a P, reference . and 2 .3-DPG. PCr. and ATP were added sequentially to
the blood to determine the relation or the chemical shi/t of blood peaks to those of
known standards (Fig. 2). There was no evidence of 2•3•DPG in the blood spectra.
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