Biodentine

26
The inflammatory process had disappeared after 15 days. The newly formed reactionary
dentine was identified. By comparison with the group treated with the glass ionomer
cement, the formation of reactionary dentine was greater in the teeth in the presence of
Biodentine and its thickness increased over time from 20 to 40 µm after 8 days, 40 to
80 µm after 15 days and 140 to 280 µm after 30 days, although it varied between 10
and 20 µm for the reference group. After 3 months, reactionary dentine generated by
Biodentine was thick and dense (Fig. 12), enclosing the horn and the mesial pulp whilst
for Fuji IX, this was less dense, only partially covering the mesial cervical area of the pulp
(Golberg, 2009).
Fig. 12. Formation of a thick reactionary dentine in presence of to Biodentine in comparison to Fuji IX
To conclude, Biodentine was able to stimulate a reactionary dentine which is a natural
barrier against bacterial invasions. The reactionary dentine formation stabilises at
3 months, indicating that the stimulation process is stopped when a sufficient dentine
barrier is formed.
5.4 - Calcification as a result of Biodentine in a direct
pulp capping and pulpotomy : pig model
Two protocols were set up in pigs (Shayegan A, 2009).
The first protocol was the analysis of the pulp reaction following pulpotomy and
placement of different materials (15 deciduous teeth, 15 pigs):
• Formocresol
• White MTA
• Biodentine
The follow-up was performed during 1, 4 and 12 weeks.
Pulp chamber was excised in 15 pigs in a comparative study of the efficacy of
Biodentine versus formocresol and MTA (5 pigs per group). Histological sections of
the teeth were done after a week, a month and 3 months of treatment. The results
showed that Biodentine, like White MTA, promoted beneficial calcification after one
week, whereas Formocresol induced necrosis and inflammation (Fig. 13).