Sedimentation, electrophoresis
Sedimentation, electrophoresis
Sedimentation, electrophoresis
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Energy<br />
energia<br />
E 2<br />
E 1<br />
to calculate<br />
c 2<br />
c 1<br />
c<br />
c<br />
c<br />
1<br />
2<br />
=<br />
e<br />
E −E<br />
1 −<br />
kT<br />
m ⎛ ρ ⎞<br />
ω ⎜ − ⎟<br />
2 ⎝ ρ ⎠<br />
2<br />
( ) 2 2<br />
r r<br />
2 m E1 − E2<br />
= 1<br />
2 −<br />
2⎛<br />
ρm<br />
⎞<br />
mω<br />
⎜1−<br />
⎟<br />
⎝ ρ ⎠<br />
( ) 2 2<br />
r −<br />
2 ln =<br />
2 r1<br />
c1<br />
2kT<br />
to measure<br />
No influence of the molecular shape!<br />
1