Sedimentation, electrophoresis

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$Microsoft PowerPoint - Intermedier filamentumok [Kompatibilis m\363d]$

F centrifug = 3. Density gradient method 2 mrω Fupthrust m = 2 ρ rω ρ m If ρ = ρ m then F c = mrω 2 = F up and v = 0! High molecular mass, small size: CsCl, CsBr, glycerol, sucrose (saccharose), ficoll Blood lipoproteins
Differential centrifugation/ cellular fractionation Albert Claude Belgian biologist (1899-1983) Nobel-prize in 1974 mitochondria microsomes lysosomes
Page 1 and 2: Sedimentation, electrophoresis Biop
Page 3 and 4: F upthrust = ρ mVg ρρ km m,
Page 5 and 6: What is centrifugal force? No accel
Page 7 and 8: Theodore Svedberg Swedish chemist (
Page 10 and 11: Velocity boundary sedimentation met
Page 12 and 13: Energy energia E 2 E 1 to calculate
Page 16 and 17: Electrophoresis Ionic double layer
Page 18: Agarose gel electrophoresis
Page 21 and 22: SDS polyacrylamide gel electrophore
Page 23 and 24: Native PAGE
Page 25 and 26: 2D electrophoresis
Page 27: iotech.szbk.u-szeged.hu/KK_Jegyzet/

Sedimentation, electrophoresis

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